具有α-葡萄糖苷酶抑制作用益生菌的筛选及特性分析

doi:10.7506/spkx1002-6630-201816028

食品科学 ›› 2018, Vol. 39 ›› Issue (16): 192-200.doi: 10.7506/spkx1002-6630-201816028

具有α-葡萄糖苷酶抑制作用益生菌的筛选及特性分析

王?芬1，刘?鹭2,3，李函彤2，张书文2，芦?晶2，逄晓阳2，汪建明1,*，吕加平2,*

（1.天津科技大学食品工程与生物技术学院，天津 300457；2.中国农业科学院农产品加工研究所，北京 100193；3.北京市营养源研究所，系统营养工程技术研究中心，北京 100069）

出版日期:2018-08-25 发布日期:2018-08-17
基金资助:
中央级公益性科研院所基本科研业务费专项（S2016JC01）

Screening for Potential Probiotics Based on High α-Glucosidase Inhibitory Activity

WANG Fen1, LIU Lu2,3, LI Hantong2, ZHANG Shuwen2, LU Jing2, PANG Xiaoyang2, WANG Jianming1,*, Lü Jiaping2,*

(1. College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China;2. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China;3. Engineering Research Centre of System-Nutrition, Beijing Institute of Nutrition Resources, Beijing 100069, China)

Online:2018-08-25 Published:2018-08-17

摘要/Abstract

摘要： 以实验室77 株益生菌为研究对象，从其菌体细胞代谢物（cell-free excretory supernatants，CFS）和细胞内容物（cell-free extracts，CFE）两方面分析菌株对α-葡萄糖苷酶的抑制活性；同时还从耐酸性、细胞黏附性等方面对具有α-葡萄糖苷酶抑制活性的菌株进行了益生特性评价；最后利用主成分分析进行综合性评价，以期筛选出具有α-葡萄糖苷酶抑制作用的益生菌。结果表明，77?株益生菌的CFE对α-葡萄糖苷酶没有抑制作用；而CFS对α-葡萄糖苷酶具有一定的抑制作用，抑制率为2.53%～15.76%。选取抑制率明显高于其他益生菌（编号为ST-2、1.1881、GS-3和BLP12）菌株进行益生特性的研究。其中ST-2表现出很高的耐酸性和细胞黏附性；GS-3在模拟消化液中有很强的耐受性等，各菌株特性不一。主成分分析表明菌株BLP12的综合性能最好：其对α-葡萄糖苷酶的抑制率可达15.10%；于pH?2.0孵育3?h后，存活率能达到71.04%；于2.0%的胆盐条件下孵育24?h，存活率为0.70%；依次经模拟唾液、胃液、肠液消化后，存活率仍能达到88.27%，但对HT-29细胞的黏附率较低，仅为1.93%，总体上菌株BLP12对体外模拟胃肠环境的适应性很强。该菌株经过16S基因序列鉴定为植物乳杆菌，可作为降糖益生菌株应用于降糖食品的开发。

关键词: α-葡萄糖苷酶, 益生菌, 抑制作用, 益生特性, 筛选

Abstract: The present study was designed to screen 77 probiotic strains based on α-glucosidase inhibitory activity of cell-free excretory supernatants (CFS) and cell-free extracts (CFE). Meanwhile, probiotic properties such as tolerance to low pH, simulated saliva (SS), simulated gastric juice (SGJ), simulated intestinal juice (SIJ), and bile salts as well as adhesion to HT-29 cells were also examined. Comprehensive evaluation was carried out by principal component analysis. Most cell-free excretory supernatants (CFS) showed α-glucosidase inhibition, ranging from 2.53% to 15.76%, but all cell-free extracts (CFE) had no α-glucosidase inhibitory activity. Strains ST-2, 1.1881, GS-3 and BLP12 showed strong ability to inhibit α-glucosidase. Strain ST-2 showed a 75.97% survival rate after 3 h of incubation at pH 2.0, and exhibited strong acid tolerance and adhesion to HT-29 cells; GS-3 showed a survival rate of 89.49% after the complete gastrointestinal transit and three strains differed in their survival rate. Principal component analysis (PCA) showed that overall, BLP12 displayed the best probiotic characteristics: its α-glucosidase inhibition rate was 15.10%; the survival rate was 71.04% after incubation at pH 2.0, 0.70% in the presence of bile salts (2.0%) for 24 h, and 88.27% after sequential digestion with SS, SGJ and SIJ; but the rate of adhesion to HT-29 cell was only 1.93%. In general, BLP12 displays strong adaptability to simulated environment. Strain BLP12 was identified as Lactobacillus plantarum by 16S rDNA sequencing. It could be used as a potential anti-diabetic probiotic strain for the development of functional foods.

Key words: α-glucosidase, probiotics, inhibition, probiotic properties, screening

中图分类号:

TS218

王芬，刘鹭，李函彤，张书文，芦晶，逄晓阳，汪建明，吕加平. 具有α-葡萄糖苷酶抑制作用益生菌的筛选及特性分析[J]. 食品科学, 2018, 39(16): 192-200.

WANG Fen, LIU Lu, LI Hantong, ZHANG Shuwen, LU Jing, PANG Xiaoyang, WANG Jianming, Lü Jiaping. Screening for Potential Probiotics Based on High α-Glucosidase Inhibitory Activity[J]. FOOD SCIENCE, 2018, 39(16): 192-200.

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具有α-葡萄糖苷酶抑制作用益生菌的筛选及特性分析

Screening for Potential Probiotics Based on High α-Glucosidase Inhibitory Activity

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