食品科学 ›› 2018, Vol. 39 ›› Issue (18): 127-132.doi: 10.7506/spkx1002-6630-201818020

• 生物工程 • 上一篇    下一篇

高通量测序分析不同产地带鱼冷藏时微生物群落多样性

高乾坤,焦琳舒,杜贺超,陆兆新,别小妹,吕凤霞*   

  1. (南京农业大学食品科学技术学院,江苏?南京 210095)
  • 出版日期:2018-09-25 发布日期:2018-09-18
  • 基金资助:
    “十二五”国家科技支撑计划项目(2015BAD16B04)

High-Throughput Sequencing Analysis of Microbial Community Diversity of Hairtail from Different Waters during Chilled Storage

GAO Qiankun, JIAO Linshu, DU Hechao, LU Zhaoxin, BIE Xiaomei, Lü Fengxia*   

  1. (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2018-09-25 Published:2018-09-18

摘要: 研究不同产地带鱼微生物群落的多样性,并探究其在冷藏过程中菌相的变化,利用高通量测序分析对产自江苏南通吕四港黄海海域和浙江舟山港东海海域的带鱼样品进行分析。结果表明:不同产地的新鲜带鱼样品菌群结构有很大差异,在属水平上,江苏南通吕四港带鱼样品中微生物主要以嗜冷菌属、栖热菌属与假交替单胞菌为主,而浙江舟山港带鱼样品中微生物菌属组成丰富,有7?种菌属的相对丰度相差不大,均为10%左右;冷藏6~8?d后,两种产地带鱼样品都以嗜冷菌属与Oceanisphaera为优势腐败菌属,两者相对丰度超过89%,对带鱼腐败变质影响显著。两种产地带鱼样品冷藏过程中细菌群落多样性及菌群结构变化,反映了带鱼在冷藏过程中潜在的卫生质量风险,可为后期带鱼冷藏过程质量安全监测及带鱼的防腐保鲜研究提供依据。

关键词: 带鱼, 高通量测序, 嗜冷杆菌属, Oceanisphaera

Abstract: To investigate the diversity and dynamics of microbial communities in hairtail from different oceans during chilled storage. high-throughput sequencing was used to analyze the microbial communities of hairtail samples from different oceans in southeast China. The results showed that there were great differences in the microbial community structures of fresh hairtail samples from different geographical regions. At the genus level, Psychrobacter, Thermus and Oceanisphaera were the dominant bacteria in the samples from Nantong. Meanwhile, a wide variety of bacterial genera were found in the samples from Zhoushan and the relative abundances of seven different genera were similar, about 10%. After being refrigerated for 6–8 days, Psychrobacter and Oceanisphaera became the dominant spoilage bacteria in the samples from both regions. The relative abundances of the two genera exceeded 89%. The microbial community diversity and microbial community structure changes during the refrigerated storage of hairtail showed potential food safety risks. The findings of this study can provide experimental data for food safety monitoring during refrigerated storage of hairtail and its preservation.

Key words: hairtail, high-throughput sequencing, Psychrobacter, Oceanisphaera

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