食品科学 ›› 2018, Vol. 39 ›› Issue (17): 221-228.doi: 10.7506/spkx1002-6630-201817036

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不同浓度β-氨基丁酸处理对葡萄果实抗病性的诱导模式

廖云霞1,费良航1,夏明星3,伍冬志1,陈 偲1,2,汪开拓1,2,*   

  1. 1.重庆三峡学院生物与食品工程学院,重庆 404100;2.南京农业大学食品科技学院,江苏 南京 210095;3.重庆出入境检验检疫局,重庆 404100
  • 出版日期:2018-09-15 发布日期:2018-09-18
  • 基金资助:
    国家自然科学基金面上项目(31671913);重庆市基础与前沿研究计划项目(cstc2015jcyjA80028);第五批重庆市高校优秀人才支持计划项目(2017019);重庆三峡学院科研创新团队建设计划项目(201703);2016年度重庆市大学生创新性实验计划项目(201610643009)

Different Modes of Disease Resistance Response Induced by β-Aminobutyric Acid at Different Concentrations in Grape Berries

LIAO Yunxia1, FEI Lianghang1, XIA Mingxing3, WU Dongzhi1, CHEN Si1,2, WANG Kaituo1,2,*   

  1. 1. College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing 404100, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 3. Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing 404100, China
  • Online:2018-09-15 Published:2018-09-18

摘要: 分析了不同浓度(1~500 mmol/L)β-氨基丁酸(β-aminobutyric acid,BABA)处理对采后葡萄果实抗病反 应的诱导作用并明确相关抗性反应的模式。结果显示,10~500 mmol/L BABA处理可有效抑制果实在20 ℃贮藏期 间灰葡萄孢(Botrytis cinerea)的侵染,从而降低果实发病率。在有效作用浓度中,经高浓度(100、500 mmol/L) BABA处理的模拟接种和B. cinerea接种葡萄果实均出现H2O2迸发、病程相关基因(VvNPR1.1、VvChit4和VvPR2) 表达量提升、抗病相关酶(几丁质酶和β-1,3-葡聚糖酶)活力升高和植保素单体(白藜芦醇和白藜芦醇脱氢二聚 体)积累等一系列典型抗病性反应。10 mmol/L BABA处理不能直接诱导果实上述抗病反应,但经10 mmol/L BABA 处理的果实在接种B. cinerea后则出现最显著的H2O2生成量、PRs转录水平和植保素含量上升现象。同时,低浓度 (10 mmol/L)BABA处理的葡萄果实中可溶性糖含量和甜度指数均显著高于高浓度(100、500 mmol/L)处理果 实。这些结果暗示,高浓度(100 mmol/L或500 mmol/L)BABA处理可诱导葡萄果实的直接抗病反应;10 mmol/L BABA处理则诱导Priming(敏化反应)作用,以使果实在病原菌侵染时展现强烈抗病性;同时,Priming抗性较直 接诱导作用可有效防止底物的过度消耗,以维持果实贮藏品质。

关键词: 葡萄, β-氨基丁酸, 诱导抗性, 模式, 品质

Abstract: The effect of β-aminobutyric acid (BABA) treatment at different concentrations ranging from 1?500 mmol/ L on the induction of disease resistance in postharvest grape berries and the resistance responses were investigated in the present study. The results showed that BABA treatment could effectively induce disease resistance against Botrytis cinerea infection in grape berries during storage at 20 ℃. Both mock-inoculated and B. cinerea-inoculated grape berries treated with 100 or 500 mmol/L BABA exhibited typical resistance responses, namely H2O2 burst, enhanced expression of defenserelated genes including VvNPR1.1, VvChit4 and VvPR2, and increased activities of chitinase and β-1,3-glucanase as well as the accumulation of stilbene phytoalexins such as trans-resveratrol and its oligomer ε-viniferin. BABA at a concentration of 10 mmol/L had no effect on inducing disease resistance in grape berries directly. However, in grape berries pretreated with 10 mmol/L BABA and then inoculated with B. cinerea, H2O2 production, the transcription levels of pathogenesis-related genes and the contents of phytoalexins significantly increased among all treatments. Meanwhile, 10 mmol/L BABA-treated grape berries maintained higher contents of soluble sugars and higher sweetness index when compared with those treated with BABA at 100 and 500 mmol/L. These results implied that high concentration of BABA (100 and 500 mmol/L) could directly activate defense responses, while low concentration of BABA (10 mmol/L) could trigger a priming defense to prevent substrate overconsumption and maintain fruit quality.

Key words: grape, β-aminobutyric acid, induced resistance, mode, quality

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