植物糖原负载提高姜黄素溶解度的研究

摘要/Abstract

摘要： 为了提高姜黄素(curcumin，CCM)表观溶解度，本文以植物糖原(phytoglycogen，PG)为载体负载CCM，制备植物糖原-姜黄素复合物（PG-CCM）；分析了体系PG浓度、CCM浓度、pH以及离子浓度对CCM表观溶解度的影响；并采用动态激光光散射法研究复合物的粒径分布，通过透射电镜对复合物的形貌进行观察，通过红外光谱、差示量热扫描分析、荧光光谱研究PG与CCM的相互作用、负载前后CCM的存在状态以及存在微环境的变化。研究结果表明：PG是CCM的高效载体，形成的PG-CCM复合物显著提高了CCM的表观溶解度。负载效果与PG和CCM浓度有关，负载体系对pH敏感，但低浓度离子对负载无显著影响。当采用10 mg/mL PG、4mg/mL CCM、pH7体系时，CCM的表观溶解度为18.68 μg/mL。复合物纳米粒的平均粒径为70~75 nm，且粒径分布均匀，呈电中性；载体PG呈现光滑的球形结构，而PG-CCM复合物表现为较大的、不规则的平面片状结构；PG-CCM复合物中的CCM以无定型非晶体结构存在，氢键是CCM与载体PG发生作用的主要作用力，CCM由极性较大的微环境向极性较小的PG作用区域转移。本文得出PG是一种高效的载体，PG-CCM复合物制备简单，可显著提高CCM的表观溶解度，有望在食品工业中拓宽CCM的应用。

关键词: 植物糖原, 姜黄素, 溶解度, 结构表征

Abstract: In order to improve the apparent solubility of curcumin (CCM), the phytoglycogen (PG) carrier was used to load CCM to prepare phytoglycogen-curcumin complex (PG-CCM). The system PG concentration was analyzed. The effect of CCM concentration, pH, and ion concentration on the apparent solubility of CCM; dynamic laser light scattering was used to study the particle size distribution of the composites, and the morphology of the composites was observed by transmission electron microscopy. The infrared spectra and differential reflectance were used to observe the morphology of the composites. Thermal scanning analysis and fluorescence spectroscopy were used to investigate the interaction between PG and CCM, the presence of CCM before and after loading, and the presence of changes in the microenvironment. The results of the study indicate that PG is an efficient carrier for CCM and the formation of PG-CCM complexes significantly increases the apparent solubility of CCM. The loading effect is related to the concentration of PG and CCM. The loading system is sensitive to pH, but low concentration ions have no significant effect on the load. When using 10 mg/mL PG, 4 mg/mL CCM, pH7 system, the apparent solubility of CCM was 29.49 μg/mL. The average particle size of the composite nanoparticles is 70-75 nm, and the particle size distribution is uniform and electrically neutral; the carrier PG presents a smooth spherical structure, while the PG-CCM compound presents a large, irregular planar sheet. The CCM in the PG-CCM complex exists in an amorphous amorphous structure. Hydrogen bonding is the main interaction between the CCM and the carrier PG. The CCM acts from a more polar microenvironment to a less polar PG. Regional transfer. This article concludes that PG is an efficient carrier and the preparation of PG-CCM compound is simple and can significantly improve the apparent solubility of CCM. It is expected to broaden the application of CCM in the food industry.

Key words: phytoglycogen(PG), curcumin (CCM), solubility, Structure Characterization

中图分类号:

TS201.1

王攀樊金玲杨亚培张月. 植物糖原负载提高姜黄素溶解度的研究[J]. 食品科学, 0, (): 0-0.

wangpan panwang 亚培杨月张. Study on the increase of curcumin solubility by phytoglycogen loading[J]. FOOD SCIENCE, 0, (): 0-0.

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