市售凉皮中金黄色葡萄球菌的肠毒素基因、耐药特征检测及SPA分型

doi:10.7506/spkx1002-6630-201820041

食品科学 ›› 2018, Vol. 39 ›› Issue (20): 287-293.doi: 10.7506/spkx1002-6630-201820041

市售凉皮中金黄色葡萄球菌的肠毒素基因、耐药特征检测及SPA分型

王盼盼1，李穗霞1，曾琪2，周陆红1，张鹏飞1，王新1,*

（1.西北农林科技大学食品科学与工程学院，陕西?杨凌 712100； 2.中国农业大学食品科学与营养学院，北京 100083）

出版日期:2018-10-25 发布日期:2018-10-24
基金资助:
国家自然科学基金面上项目（31271858）；中国博士后科学基金项目（2015M582711）

Toxin Gene Profiles, Antimicrobial Resistance, and Spa Typing of Staphylococcus aureus Isolated from Commercial Cold Noodles

WANG Panpan1, LI Suixia1, ZENG Qi2, ZHOU Luhong1, ZHANG Pengfei1, WANG Xin1,*

(1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)

Online:2018-10-25 Published:2018-10-24

摘要/Abstract

摘要： 为监测并研究凉皮中金黄色葡萄球菌的污染情况，选取连锁超市、流动摊点、小餐馆和学校餐厅4?种销售渠道，在陕西杨凌地区进行为期一年的凉皮跟踪采样，共得样本432?个，金黄色葡萄球菌检出率为24.3%（105/432）。检测sea、seb、sec、sed、see、seg、seh、sei、sej?9?种肠毒素基因，sea携带率最高为96.2%（101/105），see次之为64.8%（68/105），还有部分seb和sec检出，检出率分别为54.3%（57/105）和49.5%（52/105），seh检出率为1.0%（1/105），sed、seg、sei、sej的检出率则均为0.0%（0/105）。研究针对青霉素、氨苄西林和苯唑西林等14?种常见药物进行耐药性实验，105?株来自阳性样本的金黄色葡萄菌全部具有耐药性，对青霉素和甲氧苄啶/磺胺甲恶唑耐受率为100.0%（105/105），对头孢哌酮、环丙沙星、万古霉素和阿米卡星均敏感。此外，多重（n≥3）耐药率高达90.5%（95/105），表型为青霉素-氨苄西林-甲氧苄啶/磺胺甲恶唑-阿莫西林/克拉维酸的耐药率最高，为61.9%（65/105）。检测105?株金黄色葡萄球菌的SPA型别，共包括4?种结果，优势型别为t701（81.9%，86/105），其次是t441（16.2%，17/105），t127和t796占比最少均为1.0%（1/105）。最终，发现该地区所售凉皮除学校餐厅外，其余3?种销售渠道的检出率均较高，且菌株具有较高的肠毒素基因携带率和耐药性，优势SPA型别为t701和t441，为相应监管部门提供一定理论指导。

关键词: 金黄色葡萄球菌, 肠毒素基因, SPA分型, 耐药性

Abstract: In order to monitor and assess the contamination level of Staphylococcus aureus in cold noodles, a total of 432 cold noodles samples were collected from supermarkets, street vendor stalls, restanuants and campus canteens in Yangling, Shaanxi Province and screened for S. aureus over one year. Of these, 105 samples (24.3%) were positive for S. aureus, and the S. aureus isolates were characterized by anti-microbial susceptibility testing and PCR for detecting nine enterotoxin genes (sea to sej). All the isolates were found positive for at lest one enterotoxin gene. The genes sea (96.2%) was detected in almost all the isolates, followed by see (64.8%), and seb and sec were also found in 57 (54.3%) and 52 (49.5%) and she in only one (1.0%) of these 105 isolates. On the other hand, sed, seg, seh, sei, and sej were not found. In addition, all the S. aureus isolates were resistant to at least one of 14 common antimicrobial agents and 90.5% of them were multiresistant to three or more anti-microbials. Resistance to penicillin and trimethoprim/sulfamethoxazole (each 100.0%) was most frequently detected, and all the isolates were sensitive to ceftriaxone, ciprofloxacin, vancomycin and amikacin. The multi-drug resistance to penicillin ampicillin, trimethoprim/sulfamethoxazole and amoxicillin/clavulanic acid (Pen-Amp-T/S-A/C) was detected in 65 (61.9%) of the 105 isolates and was identied to be the most predominant. Morever, all the isolates were spa typed. A total of 4 different spa types were detected among the 105 S. aureus isolates. The results showed that S. aureus spa-t701 (81.9%) was the most predominant clone, followed by spa-t441 (16.2%),while spa-t127 and spa-t796 were the lowest (each 1.0%). In summary, this study indicates that S. aureus isolated from cold noodles in chain supermarket, street food stalls, and strains have higher drug resistance and probability to carry enterotoxin genes. Morever, the dominant SPA types are t701 and t441. Thus, these findings can provide some theoretical guidance to regulatory authorities concerned.

Key words: Staphylococcus aureus, enterotoxin genes, spa typing, antimicrobial susceptibility

中图分类号:

TS207.4

王盼盼，李穗霞，曾琪，周陆红，张鹏飞，王新. 市售凉皮中金黄色葡萄球菌的肠毒素基因、耐药特征检测及SPA分型[J]. 食品科学, 2018, 39(20): 287-293.

WANG Panpan, LI Suixia, ZENG Qi, ZHOU Luhong, ZHANG Pengfei, WANG Xin. Toxin Gene Profiles, Antimicrobial Resistance, and Spa Typing of Staphylococcus aureus Isolated from Commercial Cold Noodles[J]. FOOD SCIENCE, 2018, 39(20): 287-293.

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市售凉皮中金黄色葡萄球菌的肠毒素基因、耐药特征检测及SPA分型

Toxin Gene Profiles, Antimicrobial Resistance, and Spa Typing of Staphylococcus aureus Isolated from Commercial Cold Noodles

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