基于高通量测序法分析冷藏小龙虾优势腐败菌

摘要/Abstract

摘要： 为揭示4℃冷藏小龙虾贮藏期间微生物菌群多样性及优势腐败菌种类，以传统培养基法作为对照，采用高通量测序技术分别鉴定小龙虾样品在鲜样期、腐败中期、腐败末期的腐败菌属，探究贮藏期间微生物菌群变化趋势。两种测序方法对比发现，高通量法检测到的菌属更具多样性，培养基法提取的腐败菌均包含在高通量测序菌属内。结果表明培养基法提取优势腐败菌共11株，其中气单胞菌（Aeromonas jandaei）致腐能力最强，弗氏柠檬酸杆菌（Citrobacter freundii）致腐能力最弱；此外，高通量测序法显示希瓦氏菌（Shewanella sp.）、肉食杆菌（Carnobacterium sp.）、环丝菌属（Brochothrix sp.）、嗜冷菌属（Psychrobacter sp.）、漫游球菌属（Vagococcus sp.）、不动杆菌属（Acinetobacter sp.）等在腐败末期时的物种丰度为90.57%。两种方法比对可以确定，小龙虾的优势腐败菌有希瓦氏菌（Shewanella sp.）、肉食杆菌（Carnobacterium sp.）、环丝菌属（Brochothrix sp.）、嗜冷菌属（Psychrobacter sp.）、漫游球菌属（Vagococcus sp.）、不动杆菌属（Acinetobacter sp.）、气单胞菌（Aeromonas jandaei）、束毛球菌属（Trichococcus sp.）等。

关键词: 小龙虾, 优势腐败菌, 培养基法, 高通量测序

Abstract: In order to reveal the microbial diversity and specific spoilage organisms (SSOs) of red swamp crayfish under 4℃ refrigerated condition, the SSOs were individually isolated using the culture dependent method in this study. And their spoilage ability was analyzed to ensure its SSOs. Meanwhile, high-throughput sequencing technology was also used to identify the bacterial species of the red swamp crayfish during its freshness, the middle and the end of corruption period, which researched the changing trend of microbial community during storage at 4℃ and compared the differences between these two methods. The results showed that there were 11 strains of SSOs by culture medium, and Aeromonas jandaei had the strongest spoilage ability while Citrobacter freundii had the weakest one. In addition, high-throughput sequencing methods revealed Shewanella sp., Carnobacterium sp., Brochothrix sp., Psychrobacter sp., Vagococcus sp. and Acinetobacter sp. shared a total of 90.57% species abundance during the end of corruption. Comparing this two methods, the results showed that the SSOs in chilled crayfish mainly include Shewanella sp, Carnobacterium sp., Brochothrix sp., Psychrobacter sp., Vagococcus sp., Acinetobacter sp., Aeromonas jandaei, Trichococcus sp., and so on.

Key words: red swamp crayfish, specific spoilage organism, culture dependent method, high-throughput sequencing

江杨阳杨水兵余海霞姚洁玉胡亚芹. 基于高通量测序法分析冷藏小龙虾优势腐败菌[J]. 食品科学, 0, (): 0-0.

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