果胶多糖水热法降解及其产物抗氧化性评价

摘要/Abstract

摘要： 摘要：本文采用水热法降解商品果胶多糖，并对其降解产物的抗氧化活性进行评价。结果表明，水热法降解果胶多糖的最优工艺条件为处理温度140 ℃、处理时间30 min、pH 6；在此条件下，果胶多糖降解产物得率达46.2%。在此基础上，采用乙醇分级沉淀法对果胶多糖水热处理液进行分离，得到三种不同分子量范围的果胶多糖降解产物（S1、S2和S3），其重均分子量分别为13.4、7.5和5.7 kDa。以商品果胶多糖和三种降解产物为研究对象，进行抗氧化性评价，结果表明，S1组分对DPPH自由基的清除率达49.8%，是商品果胶的4倍；S3组分对超氧阴离子的清除率达58.7%，是商品果胶的10倍。说明水热降解果胶多糖可显著提高其抗氧化活性，为果渣废弃物的高效利用提供理论依据。

关键词: 关键词：果胶多糖, 水热法, 降解产物, 乙醇分级分离, 分子量, 抗氧化活性

Abstract: Abstract:In this work, hydrothermal treatment was carried out on commercial pectic polysaccharides, and the antioxidant activities of the degradation products from pectin polysaccharide were investigated. The results showed that the optimal conditions for pectic polysaccharides degradation were as follows: 140 oC of treatment temperature, 30 min of treatment time, and pH 5.5. Under these conditions, the yield of pectic polysaccharide degradation products was 46.2%. On this basis, three kinds of pectic polysaccharide degradation products (S1, S2 and S3) with different molecular weight ranges were isolated by ethanol fractionation method. The weight average molecular weights of pectic polysaccharide degradation products were 13.4, 7.5 and 5.7 kDa, respectively. And the antioxidant activities of pectic polysaccharides and three degradation products were evaluated. It showed that the DPPH free radical scavenging rate of S1 component could reach 49.8%, which was 4 times as high as that of commercial pectin; while the superoxide anion scavenging rate of S3 component reached 58.7%, which was 10 times as high as that of commercial pectin. It is indicated that the degradation of pectin polysaccharides by hydrothermal degradation can significantly improve its antioxidant activity, which provides a theoretical basis for the efficient utilization of pomace wastes.

Key words: Key words: pectic polysaccharide, hydrothermal treatments, degradation product, ethanol fractionation, molecular weight, antioxidant activities

中图分类号:

TS201.1

姜美云勇强唐硕王婷赖晨欢范一民. 果胶多糖水热法降解及其产物抗氧化性评价[J]. 食品科学, 0, (): 0-0.

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