猕猴桃果实中多酚类物质的分离鉴定及抗氧化活性研究

摘要/Abstract

摘要： 采用高速逆流色谱法（high-speed countercurrent chromatography, HSCCC）与制备型高效液相色谱法联用技术，对猕猴桃果实中多酚类化合物进行分离，共获得9个化合物。利用波谱方法鉴定出7个化合物结构，分别为：新绿原酸（1）、咖啡酸-3-O-葡萄糖苷（2）、2-O-咖啡酰基苏糖酸（3）、表儿茶素（5）、原花青素C1（6）、Malaxinic acid（7）、槲皮苷（8）。化合物4经初步推测可能为B-型原花青素二聚体。其中化合物3、7为猕猴桃中首次发现。化合物2、5、6、7对1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl, DPPH）和2,2’-联氮-二-（3-乙基-苯并噻唑-6-磺酸）二铵盐（2,2’-amino-di(3-ethyl-benzothiazoline-6-sulphonic acid)ammonium salt, ABTS）自由基都具有有效的清除效果，清除能力强弱为：原花青素C1>表儿茶素>咖啡酸-3-O-葡萄糖苷>Malaxinic acid。

关键词: 猕猴桃, 多酚, 分离鉴定, 抗氧化活性

Abstract: The high-speed countercurrent chromatography (HSCCC) and preparative high performance liquid chromatography were used to separate polyphenols from kiwifruit. A total of 9 compounds were obtained, and 7 compound structures were identified by spectroscopy: neochlorogenic acid (1), caffeic acid-3-O-glucoside (2), 2-O-caffeoylthreonic acid (3), epicatechin (5), procyanidin C1 (6), Malaxinic acid (7), quercitrin (8). Compound 4 may be presumed to be a B-type proanthocyanidin dimer. Among them, compounds 3 and 7 were isolated from kiwifruit for the first time. Compounds 2、5、6、7 have effective scavenging effects on DPPH and ABTS free radicals, the ability of them to remove DPPH and ABTS free radicals is: proanthocyanidin C1> epicatechin> caffeic acid-3-O-glucoside> Malaxinic acid.

Key words: kiwifruit, polyphenols, Isolation and Identification, Antioxidant Activity

中图分类号:

TS255.3

张云卜范文陆英徐海汤佳乐尹春峰刘芳林文力. 猕猴桃果实中多酚类物质的分离鉴定及抗氧化活性研究[J]. 食品科学, 0, (): 0-0.

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