耐热木聚糖酶菌株的筛选鉴定及发酵条件研究

摘要/Abstract

摘要： 因耐热木聚糖酶具有耐受环境高温、热稳定性好及应用范围广等特点，使其成为生物技术研究领域的新热点。本文从山东淄博酒曲中筛选得到一株产耐热木聚糖酶菌株FSD0302，经鉴定为疏绵状丝孢菌（Thermomyces lanuginosus）。对该菌进行Step-by-step单因素优化产酶条件考察，结果显示：当玉米芯木聚糖粒度为20-40目，浓度为4%（w/v），初始培养基pH为6.0，发酵温度50 ℃，转速200 rpm/min时，Thermomyces lanuginosus FSD0302所产木聚糖酶活力高达5357.1 U/mL，蛋白含量为10504.1 mg/mL，较初筛时产酶量提高了3.7倍；该菌可产两种木聚糖酶，分子量分别约为20和60 kDa，粗酶酶学性质考察结果表明该菌所产木聚糖酶的最适温度为75 ℃，最适pH分别为5.5及7.5且在3.5-9.0范围内可保留50%以上酶活力。综上所述，Thermomyces lanuginosus FSD0302耐热木聚糖酶可在低聚木糖生产中具有一定的应用前景。

关键词: 筛选, 鉴定, 耐热木聚糖酶, 发酵条件, 酶学性质

Abstract: Xylanase has become a new hotspot in the field of biotechnology because of its high temperature tolerance, good thermal stability and wide range of applications. And this research screened a thermostable strain FSD0302 produced xylanase from Jiuqu of Zibo, Shandong Province and it had been identified as Thermomyces lanuginosus. In order to improve the production of xylanase, the step-by-step single factor experiment had been carried out, and the results showed that when the corncob xylan particle size was 20-40 mesh, xylan concentration was 4% (w/v), initial medium pH was 6.0, fermentation temperature was 50 C, and the rotational speed was 200 rpm/min, the xylanase activity of Thermomyces lanuginosus FSD0302 reached 5357.1 U/mL and its protein content was 10504.1 mg/mL. This production was increased by 3.7 times than initial screening. Thermomyces lanuginosus FSD0302 produced two kinds of xylanases with different molecular weight of about 20 and 60 kDa, respectively. The enzymatic properties of crude xylanase showed that the optimum temperature was 75 C, the optimum pH was 5.5 and 7.5 respectively, and the activity of xylanase could be retained over 50% in the pH range of 3.5-9.0. To sum up, Thermomyces lanuginosus FSD0302 thermostable xylanase has an application prospect in the production of xylooligosaccharides.

Key words: Screening, Identification, Thermostable Xylanase, Fermentation Conditions, Enzymatic Properties

中图分类号:

Q814

杨然张笑雨刘朋肖范光森李秀婷. 耐热木聚糖酶菌株的筛选鉴定及发酵条件研究[J]. 食品科学, 0, (): 0-0.

然杨 Xiu-Ting LI. Screening and identification of thermostable xylanase-producing strain and optimizing conditions for enzyme production[J]. FOOD SCIENCE, 0, (): 0-0.

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