关键词: 花鲈, 无水活运, 暂养, 保护液, 生姜, 溶菌酶, 山梨糖醇

Abstract: The ecological ice temperature and waterless transport technology with different concentration of protective liquid in the processes of temporary aquaculture and waterless transport were proposed by testing the blood biochemical indexes of gill tissue Na+ /K+-ATPase (NKA), serum lysozyme (LZM) activity and immunoglobulin M (Ig M) level of sea perch. The results showed the perch could be best transported by the method: C2 (30 mg/L) vitamin C was added to the temporary aquaculture water with 12 h. After cold acclimation, the perch was immersed in G2(0.09% lysozyme + 0.3% sorbitol + 0.08% ginger liquor) for 2～3 s before independent packaging. In the process of temporary aquaculture and transportation with protection solution, the total protein and albumin in the blood of the perch were increased at first and then decreased, and the content of uric acid, creatinine, blood sugar and lactic acid increased significantly; The concentration of Na+ /K+-ATPase (NKA) in the gill tissue was significantly affected by the temporary aquaculture water added with Vc. But the effect on the level of immune globule M (Ig M) was not obvious. The lysozyme activity in the serum of the perch was significantly increased if the protective liquid was added with ginger. Different lysozyme activity can be caused by different doses of ginger in the preservative solution. The processes of temporary aquaculture and waterless transport combined with protective liquid can enhance the function of liver, kidney and energy metabolism, maintain normal ion activity of the gill tissue, and enhance the immune function and anti-infection ability of perch. The use of living-preserving fluid increased the mortality rate of sea perch without water transport by 1% ～5%.

Key words: Lateolabrax maculatus, waterless transport, temporary aquaculture, protection liquid, Zingiber officinale, lysozyme, sorbitol