昌黎产区酒类酒球菌（Oenococcus oeni）的遗传多样性及系统发育研究

摘要/Abstract

摘要： 摘要：截至目前，关于酒类酒球菌（Oenococcus oeni，O. oeni）遗传进化规律的研究，多集中在国外发达的葡萄酒产区，而对我国不同葡萄酒产区O. oeni遗传发育规律的深入研究较为匮乏。本研究对我国昌黎产区5家具有代表性酒庄进行O. oeni菌株的分离，采用Species-specific PCR鉴定O. oeni，运用荧光标记扩增片段长度多态性（Amplified Fragment Length Polymorphism，AFLP）技术进行分子遗传学研究。结果表明：在分离具有表型差异的226株葡萄酒乳酸菌中，通过Species-specific PCR，鉴定出222株O. oeni。对分离的O. oeni菌株进行荧光标记AFLP分析，结果显示，经筛选的荧光标记选择性扩增引物可将222株O. oeni分为221个AFLP遗传型，其相似性系数在74～98%。在81.7%的相似性水平上，分离菌株存在2个主要的进化类群。极其重要的是，分离自同一酒庄的O．oeni菌株种群形成了独特的簇群结构，呈现出遗传发育和分离起源的典型特异性关系，证实了O．oeni资源是葡萄酒“风土”的重要组成之一。本研究证实了昌黎产区存在丰富的O．oeni资源，且不同酒庄分离的O．oeni菌株种群呈现出遗传特异性，这为开发我国具有地域特色的O．oeni资源提供了技术和理论支持。

关键词: 葡萄酒, 酒类酒球菌, 扩增片段长度多态性（AFLP）, 遗传多样性, 遗传特异性, 葡萄酒“风土”

Abstract: Abstract: Up to now, the studies on genetic evolutionary of Oenococcus oeni (O. oeni) have focused on developed wine-making regions abroad, while the research on different wine-making region of China is still scarce. In this study, 222 O. oeni strains isolated from 5 different wineries in Changli region were identified by Species-specific PCR, and Amplified Fragment Length Polymorphism (AFLP) technique was used for genetics research. The AFLP results showed that there were 221 AFLP-genotypes in 222 strains with different phenotypes, and their similarity coefficient was 74～98%, revealing a high level of genetic diversity. Based on the unweighted pair-group method with Arithmetic means (UPGMA), two major phylogroups were deciphered at 81.7% similarity level. And more importantly, the strains from the same winery formed the unique clusters, indicated the presence of distinct genetic relationships with their origins, validating that O. oeni is also part of the terroir. This study confirmed that there is a rich O. oeni resource with genetic specificity in wine-making region of Changli, offering technical and theoretical support for the development of indigenous starter cultures with regional characteristics.

Key words: wine, Oenococcus oeni, amplified fragment length polymorphism, genetic diversity, genetic specificity, terroir

中图分类号:

TS261.1

余东亮石侃孟强刘树文何玲. 昌黎产区酒类酒球菌（Oenococcus oeni）的遗传多样性及系统发育研究[J]. 食品科学, 0, (): 0-0.

Dong-Liang YU HE Ling. Genetic Diversity and Phylogeny of Oenococcus oeni Strains Isolated from Wine-making Region of Changli[J]. FOOD SCIENCE, 0, (): 0-0.

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