食品科学 ›› 2019, Vol. 40 ›› Issue (1): 137-142.doi: 10.7506/spkx1002-6630-20170704-051

• 营养卫生 • 上一篇    下一篇

黑灵芝多糖对免疫抑制小鼠肠道黏膜形态及肠道黏膜免疫的影响

赵明明1,余 强1,王 辉2,向全丹1,聂少平1,谢明勇1,*   

  1. 1.南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047;2.南昌大学生命科学学院,江西 南昌 330047
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    国家自然科学基金地区科学基金项目(81760737);江西省自然科学基金资助项目(20161BAB214161);食品科学与技术国家重点实验室目标导向资助项目(SKLF-ZZA-201611)

Effect of Ganoderma atrum Polysaccharide on Intestinal Mucosal Morphology and Immunity in Immunosuppressed Mice

ZHAO Mingming1, YU Qiang1, WANG Hui2, XIANG Quandan1, NIE Shaoping1, XIE Mingyong1,*   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. College of Life Sciences, Nanchang University, Nanchang 330047, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 目的:探究黑灵芝多糖对免疫抑制小鼠肠道黏膜形态及肠道黏膜免疫的影响。方法:腹腔注射环磷酰胺构建免疫抑制小鼠模型,将小鼠分为6 组:正常对照组、模型组、阳性对照组以及黑灵芝多糖低、中、高剂量(25、50、100 mg/(kg·d))组,灌胃期间每天记录小鼠体质量并观察其生活状态,空肠组织进行切片病理学观察,酶联免疫吸附实验测定肠道白细胞介素(interlrukin,IL)-6、IL-10、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)及干扰素-γ(interferon-γ,IFN-γ)的含量,反转录定量聚合酶链式反应分析T-bet、GATA-3 mRNA相对表达水平。结果:与模型组相比,低、中、高剂量黑灵芝多糖有助于体质量回升,改善肠道黏膜形态结构,提高IL-6、IL-10、TNF-α、IFN-γ细胞因子含量,增强T-bet、GATA-3 mRNA相对表达水平,使Th1/Th2平衡向Th1偏移。结论:黑灵芝多糖可改善免疫抑制小鼠的肠道黏膜形态,调节免疫抑制小鼠的肠道黏膜免疫。

关键词: 黑灵芝多糖, 肠道黏膜, 免疫调节

Abstract: Objective: To study the effect of Ganoderma atrum polysaccharide (GAP) on intestinal mucosal morphology and immunity in immunosuppressed mice. Methods: A mouse model of immunosuppression was established by intraperitoneal injection of cyclophosphamide for 3 consecutive days. The mice were randomly divided into 6 groups: normal control, model, low-, medium- and high-dose (25, 50 and 100 mg/(kg·d)) GAP, and positive groups. Body mass was recorded and the status of the mice was observed every day. The mice were sacrificed after administration for 7 days. The mucosal morphology of the jejunum was observed by optical microscopy after hematoxylin-eosin (HE) staining. The enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). Reverse transcription-quantitative polymerase chain reaction (RTqPCR) was used to analyze the relative mRNA expression of T-bet and GATA-3. Results: Compared with the model group, administration of GAP at all three doses increased body mass, improved intestinal mucosal morphology, augmented the levels of IL-6, IL-10, TNF-α and IFN-γ, elevated the relative mRNA expression levels of T-bet and GATA-3, and shifted the Th1/Th2 balance to Th1. Conclusion: GAP can improve intestinal mucosal morphology and regulate intestinal mucosal immunity in immunosuppressed mice.

Key words: Ganoderma atrum polysaccharide, intestinal mucosa, immunomodulation

中图分类号: