食品科学 ›› 2019, Vol. 40 ›› Issue (1): 190-195.doi: 10.7506/spkx1002-6630-20170719-376

• 营养卫生 • 上一篇    下一篇

蔷薇红景天低聚原花青素对动脉粥样硬化大鼠肝脏的保护作用

韩 雪,周 茜,牛佳卉,吴梦颖,王亚旭,袁 静,赵 文*   

  1. 河北农业大学食品科技学院,河北 保定 071001
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    河北省高等学校科学技术研究项目(QN2015198);河北省食品科学和工程学科“双一流”建设资金项目(2016SPGCA18);河北省现代农业产业技术体系生猪创新团队项目(HBCT2018110205)

Hepatoprotective Effect of Oligomeric Procyanidins from Rhodiola rosea L. in Atherosclerotic Rats

HAN Xue, ZHOU Qian, NIU Jiahui, WU Mengying, WANG Yaxu, YUAN Jing, ZHAO Wen*   

  1. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 目的:研究蔷薇红景天低聚原花青素(oligomeric procyanidins from Rhodiola rosea L.,OPCRR)对动脉粥样硬化(atherosclerosis,AS)大鼠肝脏的保护作用。方法:采用VD3结合高脂乳剂灌胃的方法建立大鼠AS模型。随机分为阴性对照组,模型对照组,OPCRR 60、120、240 mg/kg mb剂量组和姜黄素(50 mg/kg mb)对照组。连续灌胃8 周后,通过分析各组大鼠肝脏的组织形态、脂质、氧化应激和炎症细胞因子等指标水平的变化,研究OPCRR对大鼠AS的干预效果。结果:与模型对照组相比,OPCRR可以显著降低大鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-c)水平(P<0.05),显著提高高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-c)水平(P<0.05),并显著降低大鼠AS指数(P<0.05);OPCRR可显著减少肝脏中的炎性灶和细胞变性,减轻肝细胞的受损程度;肝组织TC、TG、LDL-c和极低密度脂蛋白胆固醇(very low density lipoprotein cholesterol,VLDL-c)水平显著下降(P<0.05),HDL-c水平显著升高(P<0.05);肝脏中丙二醛的含量显著减少,超氧化物歧化酶和谷胱甘肽过氧化酶的活力显著提高(P<0.05);同时肝脏中的白细胞介素(interleukin,IL)-1β、IL-6、IL-10、肿瘤坏死因子-α、单核细胞趋化蛋白-1和细胞间黏附因子-1水平显著下降(P<0.05)。结论:OPCRR可以通过改善肝脏中脂质代谢、氧化应激和炎症反应而起到对AS大鼠肝脏的保护作用,且整体效果优于姜黄素。

关键词: 低聚原花青素, 蔷薇红景天, 动脉粥样硬化, 肝脏

Abstract: Objective: To study the hepatoprotective effect and underlying mechanism of oligomeric procyanidins from Rhodiola rosea L. (OPCRR) in rats with atherosclerosis (AS). Methods: A rat model of AS was established by orally administering vitamin D3 and a high-fat emulsion simultaneously. The animals were divided into negative control, AS model, positive control (curcumin at 50 mg/kg mb), and low-, medium- and high-dose OPCRR (60, 120 and 240 mg/kg mb) groups. After administration for 8 consecutive weeks, we determined the changes in liver histology, lipids, oxidative stress and inflammatory cytokines in order to evaluate the effectiveness of OPCRR in alleviating AS. Results: Compared with the AS model group, OPCRR significantly reduced the serum levels of total cholesterol (TC), triglyceride (TG), and low density lipoprotein cholesterol (LDL-c), and significantly increased serum high density lipoprotein cholesterol (HDL-c) level (P < 0.05). Meanwhile, OPCRR significantly reduced AS index, and it improved the morphology of liver cells, protected the structure of hepatic lobules, significantly reduced the number of inflammatory foci and cell degeneration in the liver, and attenuated liver cell damage. Hepatic TC, TG, LDL-c and very low density lipoprotein cholesterol levels dropped significantly, and HDL-c significantly increased in the OPCRR-treated rats compared with the model control group (P < 0.05); malondialdehyde level in the liver significantly reduced, and the activities of superoxide dismutase and glutathione peroxidase increased significantly (P < 0.05). At the same time, the levels of hepatic inflammatory cytokines (interleukin (IL)-1b, IL-6, IL-10, tumor necrosis factor-α, monocyte chemoattractant protein-1 and intercellular cell adhesion molecule-1) significantly decreased (P < 0.05). Conclusion: OPCRR can protect the liver of AS rats by regulating lipid metabolism, oxidative stress and inflammation in the liver, and its effect is better than that of curcumin.

Key words: oligomeric procyanidins, Rhodiola rosea L., atherosclerosis, liver

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