食品科学 ›› 2012, Vol. 33 ›› Issue (8): 24-28.doi: 10.7506/spkx1002-6630-201208006

• 工艺技术 • 上一篇    下一篇

纤维素酶- 微波辅助提取软枣猕猴桃茎黄酮的工艺优化

张春红,许 宁,吴 双,刘长江*   

  1. 沈阳农业大学食品学院
  • 出版日期:2012-04-25 发布日期:2012-03-31
  • 基金资助:
    农业部公益性行业科研专项(200903013)

Optimization of Cellulase Pretreatment for Microwave-Assisted Extraction of Flavonoids from Actinidia arguta Stem#br#

ZHANG Chun-hong,XU Ning,WU Shuang,LIU Chang-jiang*   

  1. (College of Food, Shenyang Agricultural University, Shenyang 110866, China)
  • Online:2012-04-25 Published:2012-03-31

摘要: 采用纤维素酶-微波辅助法提取软枣猕猴桃茎中的黄酮类物质。通过单因素试验确定纤维素酶的添加量、酶解时间、作用温度、pH值的适宜水平,在此基础上,采用响应曲面法,通过方差分析建立数学模型,得到软枣猕猴桃茎黄酮提取的最佳工艺条件:纤维素酶添加量438U/g、酶解时间64min、作用温度49℃、pH4.7,在此条件下软枣猕猴桃茎黄酮提取率为2.89%。

关键词: 软枣猕猴桃茎, 黄酮类物质, 纤维素酶, 微波, 响应面分析

Abstract: Cellulase pretreatment before microwave-assisted extraction of flavonoids from Actinidia arguta stem was optimized by response surface methodology. One-factor-at-a-time experiments were performed to select appropriate levels of enzyme dose, hydrolysis time, temperature and pH. Further, a mathematical model was built and subjected to variance analysis. The optimal cellulase hydrolysis conditions were determined as follows: the optimal enzyme dose was 438 U/g for 64 min of hydrolysis at 49 ℃ and pH 4.7. Under these conditions, the extraction rate of flavonoids was up to 2.89%.

Key words: Actinidia arguta stem, flavonids, cellulose, microwave, response surface methodology

中图分类号: