食品科学 ›› 2012, Vol. 33 ›› Issue (8): 85-88.doi: 10.7506/spkx1002-6630-201208018

• 工艺技术 • 上一篇    下一篇

响应面法优化丰年虫卵壳脱乙酰度壳聚糖提取工艺

黄晓燕,张建新*,王 欣,彭鑫华,聂 路   

  1. 西北农林科技大学食品科学与工程学院
  • 出版日期:2012-04-25 发布日期:2012-03-31
  • 基金资助:
    国家级大学生创新项目(2010A19)

Optimization of Extraction Process for Highly Deacetylated Chitosan from Chirocephalus diaphanous Eggshell by Response Surface Methodology

HUANG Xiao-yan,ZHANG Jian-xin*,WANG Xin,PENG Xin-hua,NIE Lu   

  1. (College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China)
  • Online:2012-04-25 Published:2012-03-31

摘要: 以丰年虫卵壳制备的甲壳素为原料,通过单因素试验和响应面设计法,确定丰年虫卵壳壳聚糖微波辅助提取技术的最佳工艺条件为微波时间33.4min、乙醇浸泡时间86min、碱液质量分数57.27%。按此工艺条件提取可获得脱乙酰度为84.12%的丰年虫壳聚糖。

关键词: 壳聚糖, 丰年虫, 响应面, 微波, 脱乙酰度

Abstract: One-factor-at-a-time method and response surface methodology were used to optimize the microwave-assisted extraction of highly deacetylated chitosan from Chirocephalus diaphanous eggshell. The optimal extraction conditions for achieving maximum degree of deacetylation of chitosan were as follows: microwave treatment time 33.4 min, ethanol soaking time 86 min, NaOH concentration 57.27%. Under these conditions, chitosan with a degree of deacetylation of 84.12% was obtained from Chirocephalus diaphanous eggshell.

Key words: chitosan, Chirocephalus diaphanous, response surface methodology, microwave, degree of deacetylation

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