食品科学 ›› 2012, Vol. 33 ›› Issue (8): 95-98.doi: 10.7506/spkx1002-6630-201208020

• 工艺技术 • 上一篇    下一篇

响应面法优化纤维素酶提取苹果渣中水溶性膳食纤维

牟建楼,王 颉*,孙剑锋   

  1. 河北农业大学食品学院
  • 出版日期:2012-04-25 发布日期:2012-03-31
  • 基金资助:
    河北省科技计划项目(11221004D)

Response Surface Methodology for Extraction Optimization of Water Soluble Dietary Fiber from Apple Pomace Using Cellulase

MU Jian-lou,WANG Jie*,SUN Jian-feng   

  1. (College of Food Science and Engineering, Agricultural University of Hebei, Baoding 071000, China)
  • Online:2012-04-25 Published:2012-03-31

摘要: 以苹果渣为原料,研究纤维素酶作用提取苹果渣中水溶性膳食纤维,通过单因素试验和响应面优化试验确定适宜的提取条件。结果表明:在纤维素酶用量0.67%、缓冲液pH5.55、酶解时间1.90h、酶解温度45℃条件下,水溶性膳食纤维提取率最高,为17.50%。

关键词: 苹果渣, 纤维素酶, 水溶性膳食纤维, 响应面

Abstract: One-factor-at-a-time method and response surface methodology were used to optimize the enzymatic extraction of water soluble dietary fiber from apple pomace with cellulase. The highest extraction rate of water soluble dietary fiber, 17.50%, was obtained under the conditions: enzyme dose 0.67%, buffer pH 5.55, hydrolysis time 1.90 h, and temperature 45 ℃.

Key words: apple pomace, cellulase, soluble dietary fiber (SDF), response surface analysis

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