关键词: 高效阴离子交换色谱, 脉冲安培检测, 奶粉, 糖, 柠檬酸

Abstract: A high performance anion exchange chromatographic-pulse amperometric detection method was established to determine glucose, sucrose and lactose in milk powder. The separation column used was METROSEP CARB 1 (150 mm × 4.0 mm) anion exchange column, and the mobile phase was 37 mmol/L NaOH. Citric acid was used to precipitate proteins from milk. The linear ranges of glucose, sucrose and lactose were 1－40, 1－50 mg/L and 1－50 mg/L with correlation coefficients of 0.9966, 0.9968 and 0.9985, respectively, and the detection limits were 0.014, 0.091 mg/L and 0.083 mg/L. The precision relative standard deviations of precision for 5 replicate determinations were in the range of 1.10%－4.96%. The recovery rates for glucose, sucrose and lactose in 4 different commercial samples were 90.13%－104.83%. The method was suitable for rapid determination of glucose, sucrose and lactose in milk powder, thanks to its short analysis time and simple pretreatment.

Key words: high performance anion exchange chromatography, pulsed amperometric detection, milk powder, sugar, citric acid