食品科学 ›› 2012, Vol. 33 ›› Issue (8): 188-190.doi: 10.7506/spkx1002-6630-201208040

• 分析检测 • 上一篇    下一篇

固相萃取-反相高效液相色谱法测定红毛丹中抑霉唑的残留动态

高智席1,江忠远1,李新发1,吴艳红1,牟青松1,敖克厚1,邓传跃2   

  1. 1.遵义师范学院化学系 2.西南大学化学化工学院
  • 出版日期:2012-04-25 发布日期:2012-03-31
  • 基金资助:
    州省遵义市科技局项目([2008]26 号);贵州省教育厅自然科学基金项目([2010]085 号)

Residue Dynamic Analysis of Imazalil in Rambutan by RP-HPLC with Solid-Phase Extraction

GAO Zhi-xi1,JIANG Zhong-yuan1,LI Xin-fa1,WU Yan-hong1,MU Qing-song1,AO Ke-hou1,DENG Chuan-yue2   

  1. (1. Department of Chemistry, Zunyi Normal College, Zunyi 563002, China; 2. School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China)
  • Online:2012-04-25 Published:2012-03-31

摘要: 建立固相萃取-反相高效液相色谱法测定红毛丹中抑霉唑的残留动态分析的方法。色谱柱为Shim-pack VP-ODS(150mm×4.6mm,5μm),检测波长229nm,流动相为甲醇-离子对溶液(75:25,V/V),流速0.85mL/min,进样量20μL。抑霉唑在15.0~960.0μg/L(r=0.9994)范围内与峰面积呈良好线性关系,检出限为5μg/L,抑霉唑回收率为86.4%~104.5%,相对标准偏差0.84%~2.87%。同时,还对抑霉唑由红毛丹皮向肉的迁移,在红毛丹体上的降解动态进行分析。该方法操作简便快速,可作为红毛丹中抑霉唑含量监测的方法。

关键词: 抑霉唑, 固相萃取, 反相高效液相色谱, 红毛丹

Abstract: A reversed-phase high performance liquid chromatographic method with solid-phase extraction was proposed for the residue dynamic analysis of imazalil in rambutan. The analyte was separated on a Shim-pack VP-ODS (150 mm × 4.6 mm, 5μm) using a mobile phased composed of a mixture at a volume ratio of 75:25 of methanol and acidic Na2H2PO4 solution approximately at pH 5 (adjusted with 85% phosphoric acid) at a flow rate of 0.85 mL/min, and detected at 229 nm. The injection amount was 20 μL. An excellent linearity of imazalil in the range of 15.0-960.0 μg/L was achieved (r =0.9994). The detection limit was 5.0 μg/L. The average spike recovery rates were in the range of 86.4%-104.5% with relative standard deviation of 0.84%-2.87%. The method was fast, simple, and applicable for the determination of imazalil residue in rambutan.

Key words: imazalil, solid-phase extraction, RP-HPLC, rambutan

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