胶体金免疫层析法和气相色谱法测定动物产品中氯霉素残留

doi:10.7506/spkx1002-6630-201208050

食品科学 ›› 2012, Vol. 33 ›› Issue (8): 229-232.doi: 10.7506/spkx1002-6630-201208050

胶体金免疫层析法和气相色谱法测定动物产品中氯霉素残留

宋红波，陈军，王栋，方琳，余文婕，储芳

江苏省产品质量监督检验研究院溧阳检测中心，溧阳市农畜水产品质量监督检验测试中心

出版日期:2012-04-25 发布日期:2012-03-31

Comparative Applications of Colloidal Gold Immunochromatographic Assay and Gas Chromatography for the Determination of Chloramphenicol Residues in Food Products of Animal Origin

SONG Hong-bo，CHEN Jun，WANG Dong，FANG Lin，YU Wen-jie，CHU Fang

(Liyang Testing Center, Institute of Supervision and Inspection Product Quality of Jiangsu Province, Liyang Agricultural, Domestic and Aquatic Products Quality Supervision and Testing Center, Liyang 213300, China)

Online:2012-04-25 Published:2012-03-31

摘要/Abstract

摘要： 对动物产品中氯霉素残留检测的胶体金免疫层析法和气相色谱法进行比较研究。向样品中分别添加0.3、0.5、1.5μg/kg三个添加水平的氯霉素标准品，用两种方法进行检测。胶体金免疫层析法对0.5μg/kg以上均能检出，气相色谱法平均回收率分别为78.1%、85.3%和88.3%，变异系数为5.3%～9.0%，最低检测限为0.3μg/kg。对108个样品用胶体金免疫层析法筛选出3个阳性样品，经气相色谱法验证皆为阳性，105个阴性样品经气相色谱法检测皆为阴性，未发现假阳性和假阴性。结果表明：胶体金免疫层析法具有快速，简便，特异，灵敏度高的特点，适合基层检验机构对动物产品中氯霉素残留现场快速筛选；气相色谱法灵敏度高，适用于阳性样品的精确定量。

关键词: 动物产品, 氯霉素, 胶体金免疫层析法, 气相色谱法

Abstract: Colloidal gold immunochromatographic assay (GICA) and gas chromatography (GC) was separately used for the analysis of chloramphenicol (CAP) residues in creatural products. Samples were spiked with 0.3, 0.5μg/kg and 1.5μg/kg CAP and determined by the methods. CAP residues at concentrations above 0.5μg/kg could be detected by GICA. The average recovery rates of GC were 78.1%, 85.3% and 88.3%, respectively with relative standard deviation (RSD) of 5.3%－9.0% and the detection limit was 0.3μg/kg. Three positive samples and other 105 samples analyzed by GICA were confirmed by GC and no false positive and negative samples were observed. Therefore, GICA is a rapid, accurate, reproducible and suitable to be used for rapid in situ screening of CAP residues in food products of animal origin, while GC is sensitive and suitable to be used for quantification of positive samples.

Key words: food products of animal origin, chloramphenicol, GICA, GC

中图分类号:

TS207.3

宋红波，陈军，王栋，方琳，余文婕，储芳. 胶体金免疫层析法和气相色谱法测定动物产品中氯霉素残留[J]. 食品科学, 2012, 33(8): 229-232.

SONGHong-bo，CHENJun，WANGDong，FANGLin，YUWen-jie，CHUFang. Comparative Applications of Colloidal Gold Immunochromatographic Assay and Gas Chromatography for the Determination of Chloramphenicol Residues in Food Products of Animal Origin[J]. FOOD SCIENCE, 2012, 33(8): 229-232.

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胶体金免疫层析法和气相色谱法测定动物产品中氯霉素残留

Comparative Applications of Colloidal Gold Immunochromatographic Assay and Gas Chromatography for the Determination of Chloramphenicol Residues in Food Products of Animal Origin

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