食品科学 ›› 2012, Vol. 33 ›› Issue (9): 16-20.doi: 10.7506/spkx1002-6630-201209004

• 基础研究 • 上一篇    下一篇

金华火腿中小肽的分离纯化及结构研究

王 娟,赵改名*,张建威,柳艳霞,李苗云,胡东华   

  1. 河南农业大学肉制品加工与质量安全控制重点实验室
  • 出版日期:2012-05-15 发布日期:2012-05-07
  • 基金资助:
    国家自然科学基金项目(30972129)

Separation, Purification and Structural Identification of Small Peptides from Jinhua Ham

WANG Juan,ZHAO Gai-ming*,ZHANG Jian-wei,LIU Yan-xia,LI Miao-yun,HU Dong-hua   

  1. (Key Laboratory of Meat Quality and Safety Control, Henan Agricultural University, Zhengzhou 450002, China)
  • Online:2012-05-15 Published:2012-05-07

摘要: 采用切流向过滤、葡聚糖凝胶Sephadex G-15过滤层析对成熟前和后熟结束时期金华火腿中小肽进行分离纯化。结合高效液相色谱-串联质谱仪进行分析,得到26种主要的小肽。其中有7种肽重现性较好,相对峰面积较高,其氨基酸序列分别为Ala-His、Pro-Leu、Ser-Gly-Val、Ser-Gly-Phe、Pro-Leu-Lys-Pro-Ala、Leu-Leu-Ala-His 和Asn-Pro-Thr,对应的相对峰面积分别为17.81%、8.62%、10.58%、5.70%、6.11%、6.12%和3.49%。

关键词: 金华火腿, 小肽, 分离纯化, 高效液相色谱-串联质谱, 氨基酸序列

Abstract: Tangential flow filtration (TFF) and Sephadex G-15 gel filtration chromatography were used for the separation and purification of low molecular weight polypeptides in Jinhua ham at the end of sun-drying and post-ripening. Twenty-six main peptides were obtained and analyzed by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Seven of them had better repeatability and higher relative peak area. The amino acid sequences were Ala-His, Pro-Leu, Ser-Gly-Val, Ser-Gly-Phe, Pro-Leu-Lys-Pro-Ala, Leu-Leu-Ala-His and Asn-Pro-Thr, and the relative peak areas 17.81%, 8.62%, 10.58%, 5.70%, 6.11%, 6.12% and 3.49%, respectively.

Key words: jinhua ham, small peptide, separation and purification, LC-MS/MS, amino acid sequence

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