食品科学 ›› 2012, Vol. 33 ›› Issue (9): 36-40.doi: 10.7506/spkx1002-6630-201209008

• 基础研究 • 上一篇    下一篇

桑黄菌胞内多糖的理化性质和体外抗氧化活性

闫景坤1,马海乐1,*,祝子坪1,郑惠华2,杨小明1,刘伟民1   

  1. 1.江苏大学食品与生物工程学院,江苏省农产品物理加工重点实验室 2.江苏安惠生物科技有限公司
  • 出版日期:2012-05-15 发布日期:2012-05-07
  • 基金资助:
    江苏高校优势学科建设工程资助项目;江苏省农产品物理加工重点实验室开放基金项目(JAPP2010-7); 江苏大学校基金资助项目(105DG129)

Physico-chemical Properties and Antioxidant Activity in vitro of Intracellular Polysaccharides from Phellinus igniarius

YAN Jing-kun1,MA Hai-le1,*,ZHU Zi-ping1,ZHENG Hui-hua2,YANG Xiao-ming1,LIU Wei-min1   

  1. (1. Jiangsu Key Laboratory of Physical Processing of Agricultural Product, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China ;2. Jiangsu Alphay Biological Technology Co. Ltd., Nantong 226009, China)
  • Online:2012-05-15 Published:2012-05-07

摘要: 研究液体发酵桑黄菌胞内多糖的理化性质和体外抗氧化活性。结果表明:经DEAE-52纤维素离子交换柱层析分离分级得到多个级分,以较大分子质量的中性多糖为主。凝胶渗透色谱分析表明桑黄菌胞内多糖的重均分子质量范围为5.7×103~6.1×106D,由葡萄糖、甘露糖和半乳糖组成,其分子物质的量比为15:4:1,多糖含量为41%,特性黏度为12mL/g。通过体外抗氧化模型,发现桑黄菌胞内多糖均能较好的清除 ·OH、O2- ·和螯合Fe2+,且对O2- ·具有明显的清除作用。

关键词: 桑黄, 胞内多糖, 理化性质, 体外抗氧化

Abstract: In this study, physicochemical properties and antioxidant activity of intracellular polysaccharides (IPs) isolated from liquid mycelial culture of Phellinus igniarius were analyzed. Crude IPs extract was separated into 8 fractions by DEAE-52 cellulose anion-exchange column chromatography, most of which were high molecular weight neutral polysaccharides. Gel permeation chromatographic (GPC) analysis showed that the average molecular weight (MW) of IPs ranged from 5.7 × 103 D to 6.1 × 106 D. The monosaccharide composition consisted of glucose, mannose, and galactose at a molar ratio of 15:4:1, the polysaccharide content in crude IPs extract was 41% and its intrinsic viscosity was 12 mL/g. IPs from Phellinus igniarius mycelia could effectively scavenge hydroxyl and superoxide anion free radicals and chelate Fe2+. Furthermore, more obvious scavenging activity was observed against superoxide anion free radicals than hydroxyl free radicals.

Key words: Phellinus igniarius, intracellular polysaccharides(IPs), physico-chemical properties, antioxidant activity in vitro

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