响应面法优化以中药为基质的灰树花菌丝体发酵培养基

doi:10.7506/spkx1002-6630-201209027

食品科学 ›› 2012, Vol. 33 ›› Issue (9): 127-130.doi: 10.7506/spkx1002-6630-201209027

响应面法优化以中药为基质的灰树花菌丝体发酵培养基

胡晓静1，谢英利1，叶群1，吴天祥2，杨海龙1,*

1.温州大学生命与环境科学学院 2.贵州大学生命科学学院

出版日期:2012-05-15 发布日期:2012-05-07
基金资助:
国家自然科学基金项目(31060272)

Optimization of Fermentation Medium Based on Chinese Medicinal Herbs for Mycelial Growth of Grifola frondosa by Response Surface Methodology

HU Xiao-jing1，XIE Ying-li1，YE Qun1，WU Tian-xiang2，YANG Hai-long1,*

(1. School of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China； 2. School of Life Science, Guizhou University, Guiyang 550025, China)

Online:2012-05-15 Published:2012-05-07

摘要/Abstract

摘要： 以山药、莲子、黑米、党参、薏苡仁、枸杞6味药食两用中药培养基的灰树花发酵，并运用Box-Behnken试验设计及响应面分析对培养基进行优化。结果表明：枸杞、党参适合于作碳源，莲子适合于作氮源；以枸杞、莲子为基质的最佳发酵培养基为：枸杞质量浓度23.5g/L、莲子质量浓度4.3g/L、葡萄糖质量浓度28.8g/L，在此条件下，灰树花菌体生物量可达16.937g/L。

关键词: 药食两用中药, 灰树花, 深层发酵, 响应面

Abstract: In order to maximize the mycelial growth of Grifola frondosa in submerged culture, the optimal carbon and nitrogen sources were screened out of 6 Chinese edible and medicinal herbs including Radix Dioscoreae oppositae, Semen Nelumbo, black rice, Radix Codonopsis pilosulae, Semen Coicis lachryma jobi, and Fructus lycii and the medium composition was optimized using Box-Behnken experimental design coupled with response surface analysis. Radix Codonopsis pilosulae and Fructus lycii were suitable for use as sole carbon sources and Semen Nelumbo was the most suitable nitrogen source. The optimized medium composition was composed of 23.5 g/L Fructus lycii, Semen Nelumbo 4.3 g/L and glucose 28.8 g/L, resulting in a mycelial biomass of 16.937 g/L.

Key words: Chinese edible and medicinal herbs, Grifola frondosa, submerged fermentation, response surface methodology

中图分类号:

胡晓静，谢英利，叶群，吴天祥，杨海龙. 响应面法优化以中药为基质的灰树花菌丝体发酵培养基[J]. 食品科学, 2012, 33(9): 127-130.

HUXiao-jing，XIEYing-li，YEQun，WUTian-xiang，YANGHai-long. Optimization of Fermentation Medium Based on Chinese Medicinal Herbs for Mycelial Growth of Grifola frondosa by Response Surface Methodology[J]. FOOD SCIENCE, 2012, 33(9): 127-130.

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响应面法优化以中药为基质的灰树花菌丝体发酵培养基

Optimization of Fermentation Medium Based on Chinese Medicinal Herbs for Mycelial Growth of Grifola frondosa by Response Surface Methodology

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