食（药）用蕈菌中高抑菌活性菌株筛选及培养基优化

doi:10.7506/spkx1002-6630-201209029

食品科学 ›› 2012, Vol. 33 ›› Issue (9): 138-143.doi: 10.7506/spkx1002-6630-201209029

食（药）用蕈菌中高抑菌活性菌株筛选及培养基优化

谢丽源1，雷万勇2，杜思蕴1，甘炳成1,*，彭卫红1

1.四川省农业科学院土壤肥料研究所 2.成都医学院生物医学系

出版日期:2012-05-15 发布日期:2012-05-07
基金资助:
四川省科技支撑计划项目(2008FZ0157)；国际科技合作与交流专项(2010DFA32080)

Screening of Strains with High Antibacterial Activity from Edible and Medicinal Fungi and Optimization of Culture Medium

XIE Li-yuan1，LEI Wan-yong2，DU Si-yun1，GAN Bing-cheng1,*，PENG Wei-hong1

(1. Institute of Soil and Fertilizer, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China； 2. Department of Biology and Medicine, Chengdu Medical College, Chengdu 610000, China)

Online:2012-05-15 Published:2012-05-07

摘要/Abstract

摘要： 对4个不同属24株食(药)用蕈菌对11株指示菌抑菌活性进行研究，通过初筛模型，从中筛选出3株功能菌，6株指示菌。对初筛菌株进一步复筛，确定功能菌株H2与枯草芽孢杆菌、沙门氏菌、蜡状芽孢杆菌为培养基优化的出发菌株和指示菌。通过响应面分析优化培养基配方，结果表明，当麦芽糖40.9g/L、蛋白胨11.2g/L、KH2PO4 2.1g/L、MgSO4 ·7H2O 1.1g/L时，H2对枯草芽孢杆菌、沙门氏菌、蜡状芽孢杆菌的抑菌活性均较理想，其抑菌圈半径分别达到3.513、4.610、8.800mm。

关键词: 食(药)用蕈菌, 抑菌活性, 筛选, 培养基, 响应面分析

Abstract: The antibacterial activities of 24 edible or medicinal fungus strains belonging to 4 genera against 11 indicator bacteria were studied. Three edible fungi with obvious antibacterial activity and 6 indicator bacterial strains were preliminarily screened. Secondary screening was carried out to pick out strain H2 and three indicator bacteria including Bacillus subtilis, Salmonella typhi, and Bacillus cereus for further research into the optimization of culture medium for strain H2 using response surface methodology based on inhibitory zone radii against the indicator bacteria. The results showed that the optimal culture medium for strain H2 was composed of 4.09% maltose, 1.12% peotone, 0.21% KH2PO4, and 0.11% MgSO4 ·7H2O. The inhibitory zone radii of the strain against B. subtilis, S. typhi and B. cereus were 3.513, 4.610 mm and 8.800 mm, respectively after culture in the medium.

Key words: edible and medicinal fungi, antibacterial activity, screening, culture medium, response surface methodology

中图分类号:

谢丽源，雷万勇，杜思蕴，甘炳成，彭卫红. 食（药）用蕈菌中高抑菌活性菌株筛选及培养基优化[J]. 食品科学, 2012, 33(9): 138-143.

XIELi-yuan，LEIWan-yong，DUSi-yun，GANBing-cheng，PENGWei-hong. Screening of Strains with High Antibacterial Activity from Edible and Medicinal Fungi and Optimization of Culture Medium[J]. FOOD SCIENCE, 2012, 33(9): 138-143.

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食（药）用蕈菌中高抑菌活性菌株筛选及培养基优化

Screening of Strains with High Antibacterial Activity from Edible and Medicinal Fungi and Optimization of Culture Medium

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