食品科学 ›› 2012, Vol. 33 ›› Issue (9): 202-205.doi: 10.7506/spkx1002-6630-201209042

• 生物工程 • 上一篇    下一篇

氨基硅烷修饰的磁性纳米粒子固定化碱性蛋白酶

李 杨1,2,江连洲1,2,*,李丹丹1,王胜男1,王 梅1   

  1. 1.东北农业大学食品学院 2.国家大豆工程技术研究中心
  • 出版日期:2012-05-15 发布日期:2012-05-07
  • 基金资助:
    黑龙江省科技攻关计划项目(GA09B401-6)

Immobilization of Alkaline Protease with Magnetic Nanoparticles Modified by Amino-Silane

LI Yang1,2,JIANG Lian-zhou1,2,*,LI Dan-dan1,WANG Sheng-nan1,WANG Mei1   

  1. (1. College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. National Research Center of Soybean Engineering and Technology, Harbin 150030, China)
  • Online:2012-05-15 Published:2012-05-07

摘要: 用3-氨丙基三乙氧基硅烷对四氧化三铁磁性纳米粒子表面进行修饰,以戊二醛作为交联剂固定化碱性蛋白酶。结果表明:在经过表面修饰的磁性纳米粒子具有较好的磁分离能力,碱性蛋白酶能有效地结合在修饰后的粒子表面。固定化酶的最佳条件:酶添加量为7000U/g、反应温度为40℃、时间为1.5h;固定后酶活力可达3352U/g,回收率为48%,在反复使用5次以后,依然能保持34.2%的酶活力。

关键词: 氨基硅烷, 磁性纳米粒子, 固定化, 碱性蛋白酶

Abstract: 3-Aminopropyl triethoxy silane was used to modify the surface of iron oxide magnetic nanoparticles in this study.Glutaraldehyde was used as the cross-linking agent to immobilize alkaline protease. The results showed that the surface of modified magnetic nanoparticles had a good capability of magnetic separation so that alkaline protease was immobilized on its surface well. The best immobilization conditions for alkaline protease were alkaline protease amount of 7000 U/g, reaction temperature of 40 ℃ and reaction time of 1.5 h. The enzymatic activity after immobilization was 3352 U/g and the activity recovery was 48%. Moreover, immobilized alkaline protease retained 34.2% of the initial activity after fifth repeated use.

Key words: amino-silane, magnetic nanoparticles, immobilization, alkali protease

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