食品科学 ›› 2012, Vol. 33 ›› Issue (9): 211-216.doi: 10.7506/spkx1002-6630-201209044

• 生物工程 • 上一篇    下一篇

一株产高粘度耐酸性黄原胶生产菌的选育及其发酵工艺的研究

李元鑫1,尹丽华2,亓 烨3   

  1. 1.山东京青农业科技有限公司 2.中轩生化有限公司 3.沈阳农业大学植物保护学院
  • 出版日期:2012-05-15 发布日期:2012-05-07

Screening of Xanthomonas campestris Producing High-Viscosity and Acid-Resistant Xanthan Gum and Its Fermentation Process

LI Yuan-xin1,YIN Li-hua2,QI Ye3   

  1. (1. Jingqing Agricultural Science and Technology Co. Ltd., Qingzhou 262513, China;2. Deosen Biochemical Ltd., Zibo 255400, China;3. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)
  • Online:2012-05-15 Published:2012-05-07

摘要: 选用黄原胶生产菌株野油菜黄单胞杆菌XG30-18,经微波辐射后再经亚硝基胍(NTG)处理筛选出一株产高黏度耐酸性黄原胶的突变菌株MW-42-NTG-6,并对其发酵条件进行初步筛选。确定其培养基以蔗糖和玉米淀粉做为混合碳源、大豆分离蛋白为氮源。通过正交试验确定最适发酵条件为接种量8%、转速240r/min、pH7.0、培养温度28℃。在此发酵条件下,产胶率最高可达到3.92%、黏度为1790cP、耐酸性测定1%黄原胶溶液pH值为4.2。

关键词: 黄单胞杆菌, 黄原胶, 亚硝基胍诱变, 微波诱变, 耐酸性, 发酵

Abstract: Xanthomonas campestris XG30-18 was radiated by microwave and then treated with nitrosoguanidine (NTG) and a mutant strain named as MW-42-NTG-6 was obtained. The strain had the ability to produce high-viscosity and acid-resistant xanthan gum. The optimal carbon and nitrogen sources for the strain were sucrose + corn starch and soybean protein isolate, respectively. The optimal fermentation conditions for production of high-viscosity and acid-resistant xanthan gum by the strain, as determined using orthogonal array design method, were inoculum amount of 8%, fermentation pH of 7.0, rotation speed of 240 r/min and fermentation temperature of 28 ℃. Under these conditions, the viscosity, yield, acid-resistant pH of xanthan gum were 1790 cP, 3.92% and 4.2, respectively.

Key words: Xanthomonas campestris, xanthan gum, NTG mutation, microwave mutation, acid resistance, fermentation

中图分类号: