响应面法优化无色素产普鲁兰糖菌株UVMU3-1培养基成分

doi:10.7506/spkx1002-6630-201209045

食品科学 ›› 2012, Vol. 33 ›› Issue (9): 217-220.doi: 10.7506/spkx1002-6630-201209045

响应面法优化无色素产普鲁兰糖菌株UVMU3-1培养基成分

李炳学1，葛海涛1，靳建忠1，徐琼1，肖亦农1，张宁2，韩晓日1

1.沈阳农业大学土地与环境学院 2.沈阳农业大学生物科学技术学院

出版日期:2012-05-15 发布日期:2012-05-07
基金资助:
国家自然科学基金项目(31000674)；国际合作项目(UNU-Kirin Fellowship Programme Follow-up Research 201101)；中国博士后科学基金面上资助项目(2012M510836)

Optimization of Medium Composition for Pullulan Production by Pigment-Free Mutant Strain UVMU3-1 by Response Surface Methodology

LI Bing-xue1，GE Hai-tao1，JIN Jian-zhong1，XU Qiong1，XIAO Yi-nong1，ZHANG Ning2，HAN Xiao-ri1

(1. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China； 2. College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China)

Online:2012-05-15 Published:2012-05-07

摘要/Abstract

摘要： 以Plackett-Burman(PB)设计结合响应面(RSM)分析法对无色素产普鲁兰突变菌株UVMU3-1发酵培养基7种营养成分配比进行优化。结果表明：葡萄糖、KH2PO4添加量显著影响普鲁兰产量。最陡爬坡试验使2个显著因素的水平取值逼近最大响应区域。中心组合设计结合RSM分析确定产普鲁兰最优培养基配比为：葡萄糖 67g/L、KH2PO4 5.18g/L、(NH4)2SO4 5g/L、NaNO3 10g/L、MgSO4 ·7H2O 0.5g/L、酵母粉 2g/L、吐温-80 10mL/L，预测最大响应值19.94g/L。实际验证普鲁兰产量19.98g/L，与预测相符，普鲁兰产量较优化前提高163%。

关键词: Pulluan, Plackett-Burman设计, 中心组合设计, 响应面

Abstract: Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize 7 medium components for pullulan production by pigment-free mutant strain UVMU3-1. The results indicated that the yield of pullulan was mainly influenced by the concentrations of glucose and KH2PO4. The components were approached to the maximum response area using steepest ascent design (SAD). Using central composite design combined with response surface methodology, the optimal medium composition was determined to be 67 g/L glucose, 5.18 g/L KH2PO4, 5 g/L (NH4)2SO4, 10 g/L NaNO3, 0.5 g/L MgSO4 ·7H2O, 2 g/L yeast extract and 10 mL/L Tween-80. The experimental yield of pullulan in the optimized medium was 19.98 g/L, which was close to the predicted value of 19.94 g/L and increased by 163% when compared with the control.

Key words: pullulan, Plackett-Burman design, response surface methodology, medium optimization

中图分类号:

TS201.3

李炳学，葛海涛，靳建忠，徐琼，肖亦农，张宁，韩晓日. 响应面法优化无色素产普鲁兰糖菌株UVMU3-1培养基成分[J]. 食品科学, 2012, 33(9): 217-220.

LIBing-xue，GEHai-tao，JINJian-zhong，XUQiong，XIAOYi-nong，ZHANGNing，HANXiao-ri. Optimization of Medium Composition for Pullulan Production by Pigment-Free Mutant Strain UVMU3-1 by Response Surface Methodology[J]. FOOD SCIENCE, 2012, 33(9): 217-220.

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响应面法优化无色素产普鲁兰糖菌株UVMU3-1培养基成分

Optimization of Medium Composition for Pullulan Production by Pigment-Free Mutant Strain UVMU3-1 by Response Surface Methodology

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