食品科学 ›› 2012, Vol. 33 ›› Issue (10): 84-86.doi: 10.7506/spkx1002-6630-201210017

• 工艺技术 • 上一篇    下一篇

有机溶剂沉淀法提取乳酸链球菌素的效果

鲁吉珂1,黎业娟1,吴霄玥1,刘 欣2,郝利民3   

  1. 1.郑州大学生物工程系 2.郑州奇泓生物科技有限公司 3.总后勤部军需装备研究所
  • 出版日期:2012-05-25 发布日期:2012-05-07
  • 基金资助:
    郑州大学引进人才项目;郑州大学全国大学生创新创业训练计划项目(121045943); 河南省教育厅科学技术研究重点项目(12B530005)

Separation of Nisin from Fermentation Broth Concentrate by Organic Solvent Precipitation

LU Ji-ke1,LI Ye-juan1,WU Xiao-yue1,LIU Xin2,HAO Li-min3   

  1. (1. Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China; 2. Zhengzhou Qihong Biotechnology Co. Ltd., Zhengzhou 450066, China; 3. Quartermaster Equipment Institute of General Logistics Department of People,s Liberation Army, Beijing 100010, China)
  • Online:2012-05-25 Published:2012-05-07

摘要: 利用水微溶性有机溶剂二氯甲烷、正丁醇,采用沉淀法从发酵浓缩液中提取乳酸链球菌素。结果表明:在有机溶剂和发酵浓缩液体积比1:1条件下,丁醇提取乳酸链球菌素纯度可达到63.32%,单步沉淀收率24.3%,二氯甲烷提取产品纯度39.96%,沉淀收率36.04%;二氯甲烷和丁醇体积比1:1组成的复合溶剂,提取效果优于单一有机溶剂,产品纯度和单步收率分别为45.94%和59.3%。该方法可克服现有乳酸链球菌素提取工艺产品纯度低、盐含量高的缺陷,具有过程简便、产品纯度较高的优点,具有较好的工业化应用前景。

关键词: 沉淀, 有机溶剂, 乳酸链球菌素, 发酵浓缩液, 分离

Abstract: To obtain high purity nisin product through simple process with low cost, nisin was precipitated from fermentation broth concentrated with organic solvents dichloromethane and n-butanol. Under 1:1 (V/V) conditions with butanol employed, the nisin purity was 63.32%, and the precipitation yield was 24.3%. While the purity and yield for dichloromethane was 39.96% and 36.04%, respectively. When a mixture of dichloromethane and butanol at a volume ratio of 1:1 was used, the nisin purity and the precipitation yield was 45.94% and 59.3%, respectively. This new method overcame many disadvantages of the existing method, such as low purity and high salt content. So it shows great potential for industrial applications.

Key words: precipitation, organic solvent, nisin, fermentation broth concentrate, separation

中图分类号: