食品科学 ›› 2012, Vol. 33 ›› Issue (10): 246-250.doi: 10.7506/spkx1002-6630-201210051

• 分析检测 • 上一篇    下一篇

HPLC法测定茶树花中可溶性糖、儿茶素和游离氨基酸

徐人杰1,王 琳1,汪名春1,叶 红1,屠幼英2,曾晓雄1,*   

  1. 1.南京农业大学食品科技学院 2.浙江大学农业与生物技术学院茶学系
  • 出版日期:2012-05-25 发布日期:2012-05-07
  • 基金资助:
    江苏省科技支撑项目(BE2010346);江苏高校优势学科建设工程项目

Determination of Soluble Sugars, Catechins and Free Amino Acids in Tea Flowers by High Performance Liquid Chromatography

XU Ren-jie1,WANG Lin1,WANG Ming-chun1,YE Hong1,TU You-ying2,ZENG Xiao-xiong1,*   

  1. (1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Department of Tea Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China)
  • Online:2012-05-25 Published:2012-05-07

摘要: 采用高效液相色谱法测定茶树(Camellia sinensis)花中可溶性糖、儿茶素和游离氨基酸的含量。结果表明:茶树花中含有216.85mg/g可溶性糖,其花冠、雄蕊群和雌蕊群部分(F-1)及花托、萼片和花梗部分(F-2)的含量分别为250.945mg/g和112.65mg/g;茶树花中总儿茶素含量为37.03mg/g,且F-2中的含量(46.90mg/g)高于F-1(30.88mg/g);茶树花中含有包括茶氨酸在内的16种游离氨基酸,含量为10.87mg/g,虽然F-2中游离氨基酸的种类较少,但其含量(16.47mg/g)显著高于F-1(10.59mg/g)。

关键词: 茶树花, 可溶性糖, 茶叶儿茶素, 游离氨基酸, 高效液相色谱法

Abstract: The contents of soluble sugars, catechins and free amino acids in tea flowers from Camellia sinensis were analyzed by high performance liquid chromatography (HPLC). The results showed that tea flowers contained 216.85 mg/g soluble free sugars. Meanwhile, the contents of soluble sugars in corolla, stamen and pistil (F-1) and receptacle, sepal and pedicel (F-2) were 250.945 mg/g and 112.65 mg/g, respectively. Tea flowers contained 37.03 mg/g catechins. In addition, the content of catechins in F-2 was 46.90 mg/g, which was higher than that in F-1 (30.88 mg/g). Tea flower contained 16 kinds of free amino acids, and the content of total free amino acids was 10.87 mg/g. Although only 13 kinds of free amino acids were detected in F-2, its content (16.47 mg/g) was notably higher than that in F-1 (10.59 mg/g).

Key words: tea flower, soluble sugar, tea catechins, free amino acid, high performance liquid chromatography (HPLC)

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