食品科学 ›› 2012, Vol. 33 ›› Issue (10): 278-281.doi: 10.7506/spkx1002-6630-201210058

• 分析检测 • 上一篇    下一篇

GPC-HPLC-FLD 法测定动植物油脂中的苯并(a)芘

马君刚,张煌涛,于 红,任水英*   

  1. 国家农副产品监督检验中心(新疆)
  • 出版日期:2012-05-25 发布日期:2012-05-07

Determination of Benzo(a) pyrene in Animal Fats and Vegetable Oils by Gel Permeation- High Performance Liquid Chromatography with Fluorescence Detector

MA Jun-gang,ZHANG Huang-tao,YU Hong,REN Shui-ying*   

  1. (National Quality Supervision Test Center of Agricultural Byproducts (Xinjiang), U .. ru..mqi 830004, China)
  • Online:2012-05-25 Published:2012-05-07

摘要: 建立动植物油脂中苯并(a)芘残留量的凝胶净化-高效液相色谱-荧光检测的测定方法。方法采用凝胶色谱净化系统处理样品,可除去油脂中甘油三酯等杂质的干扰。色谱条件:Hypersil ODS2(150mm×4.6mm,5μm)反相色谱柱,流动相为甲醇-水(94:6,V/V),流速1.0mL/min,激发波长365nm,发射波长406nm,柱温30℃。方法的检出限0.1μg/kg,线性范围0.1~50μg/kg,加标回收率92.0%~106.0%,相对标准偏差为1.86%(n=6)。该法具有样品预处理简单、灵敏度高、精密度高等优点,可用于动植物油脂样品中苯并(a)芘的快速准确测定。

关键词: 苯并(a)芘, GPC净化系统, 高效液相色谱, 荧光检测, 动植物油脂

Abstract: This paper describes a method to determine benzo(a) pyrene in animal fats and vegetable and oils using gel permeation-high performance liquid chromatography with fluorescence detector (HPLC-FLD). In this method, gel permeation cleanup system was used to remove the interference such as triglyceride. Chromatographic separation was achieved by a reverse-phase column of Hypersil ODS2 with the mobile phase consisting of methanol-water (94:6, V/V) at a flow rate of 1.0 mL/min. FLD were set at 365 nm excitation and 406 nm emission wavelengths, and the column temperature was kept at 30 ℃. The limit of detection was 0.1μg/kg, the calibration curve was linear between a concentration range of 0.1-50μg/kg, the recoveries were in the range of 92.0%-106.0%, and RSD (n = 6) value was 1.86%. The method is simple, rapid, accurate and sensitive for directly determining benzo(a)pyrene in animal fats and vegetable oils without complicated and time-consuming separation process.

Key words: benzo(a)pyrene, GPC cleanup system, high performance liquid chromatography (HPLC), fluorescence detection (FLD), animal fats and vegetable oils

中图分类号: