食品科学 ›› 2012, Vol. 33 ›› Issue (10): 298-300.doi: 10.7506/spkx1002-6630-201210062

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采后钙处理对香菇多糖和细胞稳定性的影响

侯 雪,李喜宏*,薛 婷   

  1. 天津科技大学食品工程与生物技术学院
  • 出版日期:2012-05-25 发布日期:2012-05-07
  • 基金资助:
    “十一五”国际科技职称计划项目(2008BADAIB07)

Effect of Postharvest Calcium Treatment on Lentinan Content and Cell Stability of Lentinula edodes

HOUXue,LIXi-hong*,XUETing   

  1. (College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China)
  • Online:2012-05-25 Published:2012-05-07

摘要: 以采后香菇子实体为原料,采取CaCl2溶液真空渗透法,研究Ca2+处理对采后香菇多糖、β-1,3-葡聚糖酶活性、膜透性和MDA的影响。结果表明:0.12mol/L CaCl2处理的效果最好,β-1,3-葡聚糖酶活性为20.54U/mg,香菇多糖含量为0.38%,膜电导率为85.11%,MDA含量为2.48μmol/L,均优于对照。不同浓度Ca2+处理均能抑制香菇采后β-1,3-葡聚糖酶活性,降低香菇多糖贮藏期间的损耗,并有效维持细胞膜结构的完整性。

关键词: 香菇, 钙, 香菇多糖, β-1, 3-葡聚糖酶

Abstract: Fruit bodies of Lentinula edodes subjected to vacuum-infiltrated calcium chloride treatment were used as the materials to explore the effect of calcium treatment on lentinan content, β-1,3-glucanase activity, electrolyte leakage and MDA content. The results showed that the effect of 0.12 mol/L CaCl2 was the best solution. Under the treatment of 0.12 mol/L CaCl2, β-1,3-glucanase activity was 20.54 U/mg, lentinan content was 0.38%, electrolyte leakage was 85.11% and MDA content was 2.48 μmol/L. Calcium treatments could restrain β-1,3-glucanase activity, reduce the loss of lentinan during storage and maintain the integrity of cell membrane structure.

Key words: Lentinula edodes, calcium, lentinan, β-1,3-glucanase

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