食品科学 ›› 2012, Vol. 33 ›› Issue (11): 57-61.doi: 10.7506/spkx1002-6630-201211013

• 基础研究 • 上一篇    下一篇

鹿蹄橐吾多糖LW21 的分离纯化及其结构分析

刘春兰1,杜 宁1,徐桂云2,黄 潇1,阿西娜1   

  1. 1.中央民族大学生命与环境科学学院 2.中国科学院化学研究所
  • 出版日期:2012-06-15 发布日期:2012-07-27
  • 基金资助:
    中央高校基本科研业务费专项资金资助项目(0910KYZY44);“111 创新引智计划”工程项目(B08044); 中央民族大学“985”工程项目(MUC985-9)

Isolation, Purification and Structural Analysis of Water-Soluble Polysaccharide LW21 from Ligularia hodgsonii

LIU Chun-lan1,DU Ning1,XU Gui-yun2,HUANG Xiao1,A Xi-na1   

  1. (1. College of Life and Environmental Science, Minzu University of China, Beijing 100081, China; 2. Institute of Chemistry Chinese Academy of Sciences, Beijing 100080, China)
  • Online:2012-06-15 Published:2012-07-27

摘要: 目的:确定鹿蹄橐吾多糖LW21的结构,为探讨鹿蹄橐吾多糖的药理活性,合理利用鹿蹄橐吾这种植物资源提供依据。方法:水提取醇沉获得鹿蹄橐吾水溶性粗多糖LW,经酸性乙醇分级和DEAE-SephdexA-25纯化得多糖LW21。纸层析、醋酸纤维薄膜电泳和Sepharose CL-4B柱层析进行纯度鉴定,LW21结构分析采用高碘酸氧化、Smith降解、甲基化分析及IR、NMR、GC和GC-MS等方法。结果表明:LW21为均一多糖,相对分子质量约为1.1×106。其单糖组成为鼠李糖(Rha)、阿拉伯糖(Ara)、甘露糖(Man)、葡萄糖(Glc)、半乳糖(Gal),物质的量比为7.4:11.9:25.7:40.0:14.9。多糖LW21为有分枝结构,主链由Glc和Man构成,其中其中Man主要以β(1→2)和β(1→6)糖苷键连接,β(1→2)糖苷键在3-O处和6-O处有分枝,β(1→6)糖苷键在2-O和4-O处有分枝,Glc也主要以β(1→2)及β(1→6)糖苷键连接,β(1→2)糖苷键在6-O处有分枝,β(1→6)糖苷键连接,在2-O处有分枝。分子支链由Ara、Rha、Gal构成。末端残基为Gal、Ara、Rha、Glc。结论:鹿蹄橐吾多糖LW21是一新结构多糖,为首次从鹿蹄橐吾中分离得到。

关键词: 鹿蹄橐吾, 多糖, 甲基化分析, 结构分析

Abstract: Objective: to isaolate, purify and structurally characterize a water-soluble polysaccharide from the whole herbs of Ligularia hodgsonii. Methods: crude polysaccharide named as LW was extracted from Ligularia hodgsonii. A ploysaccharide fraction named as LW21 was obtained after acidic ethanol fractionation and DEAE-Sephadex A-25 gel filtration. The purity of LW21 was identified by paper chromatography, Sepharose CL-4B chromatography and cellulous acetate electrophoresis, and its structure was analyzed by HIO4 oxidation, Smith degradation, methylation, IR, NMR, GC and GC-MS. Results: LW21 was a homogenous polysaccharide. LW21 was composed of rhamose (Rha), arabinose (Ara), mannose (Man), glucose (Glc) and galactose (Gal) with a molar ratio of 7.4: 11.9: 25.7: 40.0: 14.9. Its average molecular weight was 110 kD. Its main chain was made up of β-(1→2)-linked or β-(1→6)-linked Man, and β-(1→2)-linked orβ-(1→6)-linked Glc residues. The β-(1→2)-linked Man was substituted at 2-O and 4-O; β-(1→6)-linked Man was substituted at 3-O and 6-O; and β-(1→2)-linked Glc was substituted at 6-O; β-(1→6)-linked Gal was substituted at 2-O. The side chain was composed of Rha, Ara and Gal. The non-reduced end was composed of Gal, Ara, Rha and Glc. Conclusion: LW21 is a new polysaccharide isolated from Ligularia hodgsonii for the first time.

Key words: Ligularia hodgsonii, polysaccharide, methylation analysis, structural analysis

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