食品科学 ›› 2012, Vol. 33 ›› Issue (11): 116-120.doi: 10.7506/spkx1002-6630-201211026

• 基础研究 • 上一篇    下一篇

魔芋粉对鲤鱼肌原纤维蛋白凝胶特性的影响

杨 振,孔保华*,夏秀芳,陈 倩,李沛军   

  1. 东北农业大学食品学院
  • 出版日期:2012-06-15 发布日期:2012-07-27
  • 基金资助:
    东北农业大学创新团队项目(CXZ011);黑龙江省教育厅海外学人科研资助项目(12511053)

Influence of Konjac Flour on Gel Properties of Myofibrillar Proteins from Common Carp

YANG Zhen,KONG Bao-hua*,XIA Xiu-fang,CHEN Qian,LI Pei-jun   

  1. (College of Food Science, Northeast Agricultural University, Harbin 150030, China)
  • Online:2012-06-15 Published:2012-07-27

摘要: 从鲤鱼背部肌肉中提取肌原纤维蛋白,分别添加0.05、0.10、0.15、0.20g/100mL的魔芋粉,研究其在不同加热温度(70、80、90℃)和不同NaCl浓度(0.05、0.10、0.15、0.20mol/L)条件下对肌原纤维蛋白凝胶的硬度、弹性、白度和保水性的影响。结果表明:相同魔芋粉添加量条件下,加热温度80℃时形成的肌原纤维蛋白凝胶的硬度和弹性显著高于70℃和90℃(P<0.05);90℃时凝胶白度高于70℃和80℃;90℃时保水性显著高于70℃时的保水性(P<0.05),与80℃的凝胶保水性差异不显著(P>0.05)。在此条件下,随着NaCl浓度增加,凝胶的硬度和弹性增大;肌原纤维蛋白凝胶的保水性显著提高。同一温度条件下,添加0.10g/100mL魔芋粉的蛋白凝胶硬度达到最大值,且80℃时硬度最大为129g,凝胶的白度随着魔芋粉质量浓度增加呈现下降趋势,保水性随着魔芋粉质量浓度的增加而增大;添加NaCl可以显著提高凝胶的白度。

关键词: 鲤鱼, 肌原纤维蛋白, 魔芋粉, 凝胶特性

Abstract: In this study, myofibrillar proteins were extracted from the back muscle of common carp and the influence of konjac flour(0.05, 0.10, 0.15, 0.20 g/100 mL), temperature (70, 80, 90 ℃) and NaCl (0.05, 0.10, 0.15, 0.20 mol/L on gel properties of the proteins, including hardness, springiness, whiteness and water-holding capacity, was investigated. Generally, the hardness and springiness of myofibrillar protein gels formed in the presence of konjac flour were higher at 80 ℃ than at 70 ℃ and 90 ℃ (P <0.05). Gel hardness and springiness also increased due to the addition of NaCl. Myofibrillar protein gels exhibited a higher whiteness at 90 ℃ compared with 70 ℃ and 80 ℃. The water-holding capacity at 90 ℃ was significantly higher than that at 70 ℃ (P<0.05), but showed no significant difference from that at 80 ℃ (P> 0.05). A significant increase in water-holding capacity was found with increasing NaCl addition. In addition, when konjac flour was added at a concentration of 0.10 g/100 mL, the highest gel strength was achieved for myofibrillar protein gels formed at the same temperature and reached 129 g at 80 ℃. As the concentration of konjac flour increased, gel whiteness decreased, while water-holding capacity increased. Moreover, the addition of NaCl resulted in a significant increase in gel whiteness.

Key words: common carp, myofibrillar protein, konjac flour, gel properties

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