食品科学 ›› 2012, Vol. 33 ›› Issue (11): 231-236.doi: 10.7506/spkx1002-6630-201211049

• 生物工程 • 上一篇    下一篇

混菌固态发酵生产菜籽肽工艺条件优化

鞠兴荣1,2,王雪峰1,何 荣1,2,王立峰2,袁 建1   

  1. 1.南京财经大学食品科学与工程学院,江苏省粮油品质控制及深加工技术重点实验室 2.江南大学食品学院
  • 出版日期:2012-06-15 发布日期:2012-07-27
  • 基金资助:
    国家农业成果转化资金项目(2009C10045);江苏省农业科技自主创新基金项目(cx(10)444;cx(10)234); 江苏省自然科学基金项目(BK2010573)

Optimization of Rapeseed Peptide Production by Mixed Solid-State Fermentation

JU Xing-rong1,2,WANG Xue-feng1,HE Rong1,2,WANG Li-feng2,YUAN Jian1   

  1. (1. Key Laboratory of Grain and Oils Quality Control and Deep-Utilizing Technology of Jiangsu Province, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210003, China; 2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
  • Online:2012-06-15 Published:2012-07-27

摘要: 以肽得率、氮溶解指数和硫苷降解率为指标,通过单因素试验初步得到枯草芽孢杆菌与雅致放射毛霉混菌固态发酵生产菜籽肽的发酵条件,再根据Box-Behnken中心组合试验设计,在单因素试验的基础上,采用响应面分析法进一步优化混菌发酵条件,确定最佳工艺条件为:枯草芽孢杆菌与雅致放射毛霉同时接种,两菌接种体积比4:1、发酵温度32.1℃、发酵时间4.64d,此条件下菜籽肽得率为9.85%,硫苷降解率为67.83%。

关键词: 混菌固态发酵, 菜籽肽, 工艺条件, 响应面分析法

Abstract: Based on peptide yield, nitrogen solubility index (NSI) and glucosinolate degradation rate, the production conditions for rapeseed peptide by mixed solid-state fermentation using Bacillus subtilis and Actinomucor elegans preliminarily determined by one-factor-at-a-a-time method and further optimized by response surface analysis coupled with Box-Behnken design. The optimal fermentation conditons were 4:1 of Bacillus subtilis-to-Actinomucor elegans ratio, 32.1 ℃ of fermentation temperature, 4.64 d of fermentation time, resulting in a peptide yield of 9.85% and a glucosinolate degradation rate of 67.83%.

Key words: mixed solid-state fermentation, rapeseed peptide, process conditions, response surface methodology

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