食品科学 ›› 2012, Vol. 33 ›› Issue (12): 18-23.doi: 10.7506/spkx1002-6630-201212004

• 工艺技术 • 上一篇    下一篇

MLM型结构脂的酶法合成及其理化性质分析

潘丽军1,2,寿佳菲1,2,符克皇1,操丽丽1,2,姜绍通1,   

  1. 1. 合肥工业大学生物与食品工程学院
    2.安徽省农产品精深加工重点实验室
  • 出版日期:2012-06-25 发布日期:2012-07-27
  • 基金资助:
    “十二五”国家科技支撑计划项目(2011BAD02B04)

Enzymatic Synthesis and Physicochemical Properties of MLM-Type Structured Lipids

PAN Li-jun1,2,SHOU Jia-fei1,2,FU Ke-huang1,CAO Li-li1,2,JIANG Shao-tong1,2   

  1. (1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China; 2. Key Laboratory for Agriculture Products Processing of Anhui Province, Hefei 230009, China)
  • Online:2012-06-25 Published:2012-07-27

摘要: 以醇解反应制得单甘酯和辛酸为底物,1,3-特异性固定化脂肪酶为催化用酶,在单因素试验的基础上,通过正交试验获得酯化反应制备MLM型结构脂质的最适条件:Lipozyme RM IM酶加入量9%(以底物质量计)、反应时间14h、反应温度40℃、单甘酯与辛酸(底物)物质的量比1:6;该条件下辛酸插入率达60.48%,其中92.84%的辛酸分布在甘油结构的1,3位上。经二级分子蒸馏纯化后得到的MLM型结构脂质,辛酸插入率达到73.34%;对其进行理化指标检测分析表明,该产品最低可达国家四级菜籽油的标准。

关键词: 结构脂质, 菜籽油, 单甘酯, 辛酸, 酯化反应

Abstract: Monoglycerides were prepared through alcoholysis and used for the synthesis of MLM-type structured lipids through its esterification with caprylic acid under the catalysis of immobilized 1,3-specific lipase. The optimal esterifcation conditions, as determined using orthogonal array design based on one-factor-at-a-time experiments, were 9% of lipozyme RM IM lipase concentration (on the basis of substrate mass), 14 h of reaction time, 40 ℃ of reaction temperature, and 1:6 of molar ratio of MAGs to caprylic acid. Under these conditions, the incorporation rate of caprylic acid was 60.48%, and 92.84% caprylic acid was distributed at the 1or 3 position of glycerol. Purified MLM-type structured lipids were obtained after subsequent two-step molecular distillation with an incorporation rate of 73.34% for caprylic acid. The physicochemical properties could comply with the national standards of Grade 4 rapeseed oil at least.

Key words: structured lipids, rapeseed oil, monoglycerides, caprylic acid, esterification

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