食品科学 ›› 2012, Vol. 33 ›› Issue (12): 58-62.doi: 10.7506/spkx1002-6630-201212012

• 工艺技术 • 上一篇    下一篇

超滤法浓缩乳清蛋白前处理研究

雷 静,李开雄*,卢士玲,刘洁洁,向艳红   

  1. 石河子大学食品学院
  • 出版日期:2012-06-25 发布日期:2012-07-27
  • 基金资助:
    石河子大学重大科技攻关项目(gxji2008zdgg04)

Pre-treatments of Whey Protein before Ultrafiltration Condensation

LEI Jing,LI Kai-xiong*,LU Shi-ling,LIU Jie-jie,XIANG Yan-hong   

  1. (College of Food Science, Shihezi University, Shihezi 832003, China)
  • Online:2012-06-25 Published:2012-07-27

摘要: 为了提高乳清在进行超滤的通透性,从而提高超滤的效率,对利用CaCl2絮凝沉淀乳清的工艺条件进行研究,通过单因素和正交试验优化,综合考虑蛋白含量、脂肪含量和透光率3个指标,确定最佳的絮凝工艺条件为pH7.5、1mol/L CaCl2溶液添加量12mL/L、在60℃水浴缓慢搅拌絮凝15min。在最佳絮凝工艺条件下进行验证,结果表明:经过预处理后的乳清蛋白损失率只有13.6%,脂肪去除率达到86%以上,透光率也有显著提高。

关键词: 干酪乳清, 絮凝, 离心

Abstract: In order to improve the permeability and ultrafiltration efficiency of whey protein, the flocculation of whey protein with CaCl2 was optimized by one-factor-at-a-time and orthogonal array design methods based on protein content, fat content and transmittance. The optimal flocculation conditions were determined as pH 7.5, 1 mol/L CaCl2 addition at a ratio of 12 mL/L, and gentle stirring on 60 ℃ water bath for 15 min. After these pretreatments, the loss rate of whey protein was only 13.6%, the defatting rate was over 86%. Meanwhile, the transmittance was significantly improved.

Key words: cheese whey, flocculation, centrifugation

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