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• 生物工程 •    下一篇

枫香拟茎点霉B3生物转化花生废弃物合成白藜芦醇的工艺

刘连红1,陈飞1,管永祥2,仇美华2,张丽1,戴传超1   

  1. 1. 南京师范大学
    2. 江苏省耕地质量保护与环境监测站
  • 收稿日期:2019-02-03 修回日期:2020-01-16 出版日期:2020-03-25 发布日期:2020-03-23
  • 通讯作者: 戴传超 E-mail:daichuanchao@njnu.edu.cn
  • 基金资助:
    江苏省重点研发计划(现代农业)项目

Synthesis of Resveratrol from Peanut Wastes by Biotransformation of Phomopsis liquidambari B3

LIU LIANHONG 2, 2, 2, 2,Chuan-Chao DAI   

  • Received:2019-02-03 Revised:2020-01-16 Online:2020-03-25 Published:2020-03-23
  • Contact: Chuan-Chao DAI E-mail:daichuanchao@njnu.edu.cn

摘要: 为探究接种枫香拟茎点霉B3对花生废弃物中白藜芦醇含量的影响,采用代谢物检测和关键酶基因的定量实验进行验证。结果表明,枫香拟茎点霉B3既可以通过分泌糖苷酶转化花生废弃物中的白藜芦醇苷为白藜芦醇,又可以通过从头合成途径利用花生废弃物中的对香豆酸生产白藜芦醇。在此基础上,采用响应面优化发酵条件对白藜芦醇转化效率的影响,得到在料液比为1:30(g/mL)[补充单位]条件下,培养基初始pH 5.2、接种量9%、发酵温度28.6 ℃、发酵转速204 rpm时,菌株转化合成白藜芦醇效率最显著。上5 L发酵罐进行中试放大实验,经过实测经过36 h白藜芦醇含量提高了190%。研究结果表明,接种枫香拟茎点霉B3可以显著提高花生废弃物中白藜芦醇含量,本研究对于花生中高附加值产物的开发和花生综合利用途径的拓宽具有重要的参考意义。

关键词: 花生废弃物, 白藜芦醇, 枫香拟茎点霉B3, 发酵, 响应面分析

Abstract: To study the effect of inoculating Phomopsis liquidambari B3 on resveratrol content in the peanut wastes, the metabolite detection and real-time quantitative PCR methods were used. The results showed that B3 could secrete β-glucosidase to translate piceid of peanut wastes to resveratrol. It could also use p-coumaric acid of peanut wastes to synthesize resveratrol by de novo synthesis. On this basis, the resveratrol conversion efficiency was optimized by investigating the effects of various factors and their interactions on reducing power using one-factor-at-a-time method and response surface methodology. A ratio of solid to liquid of 1:30, an initial pH value of 5.2 for medium, an inoculation volume of 9%, a fermentation temperature of 28.6 ℃ and a rotating speed of 204 rpm were found to the optimal conditions to obtain a higher yield of reveratrol. The content of resveratrol in the 5 L fermentation tank was increased 190% after 36 h. The results showed that the application of P. liquidambari B3 could significantly increase the content of resveratrol in the peanut wastes, which is beneficial to the development of products with high added value in peanuts and the widen of comprehensive utilization ways of peanuts.

Key words: peanut wastes, resveratrol, Phomopsis liquidambari B3 , fermentation, response surface analysis

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