食品科学 ›› 2019, Vol. 40 ›› Issue (8): 270-274.doi: 10.7506/spkx1002-6630-20180617-340

• 安全检测 • 上一篇    下一篇

利用蛋白组学和化学计量学区分海捕和养殖的中国对虾

胡玲萍,张鸿伟,张 峰,林 超,薛长湖,张晓梅   

  1. 1.中国海洋大学食品科学与工程学院,山东 青岛 266003;2.山东出入境检验检疫技术中心,山东 青岛 266002;3.中国检验检疫科学研究院,北京 100176
  • 出版日期:2019-04-25 发布日期:2019-05-05
  • 基金资助:
    国家自然科学基金联合基金项目(U606403);国家自然科学基金面上项目(31571864);国家质量监督检验检疫总局科技计划项目(2016IK204)

Discrimination of Wild and Farmed Fenneropenaeus chinensis Using Proteomics and Chemometrics

HU Lingping, ZHANG Hongwei, ZHANG Feng, LIN Chao, XUE Changhu, ZHANG Xiaomei   

  1. 1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; 2. Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China; 3. Chinese Academy of Inspection & Quarantine, Beijing 100176, China
  • Online:2019-04-25 Published:2019-05-05

摘要: 使用超高效液相色谱-飞行时间质谱连续窗口采集所有理论碎片离子技术为基础的蛋白组学,对海捕和养殖中国对虾的蛋白进行相对定量,并通过化学计量学寻找区分海捕和养殖中国对虾的蛋白生物标志物。结果表明,筛选所得的蛋白生物标志物可以很好地区分海捕和养殖的中国对虾。以期为海捕和养殖中国对虾的区分提供一定的技术支持。

关键词: 中国对虾, 海捕, 养殖, 蛋白组学, 化学计量学

Abstract: In this study, sequential window acquisition of all theoretical fragment ions (SWATH)-based proteomics was established for relative quantitation of proteins by ultra-high performance liquid chromatography coupled to quadrupole time of flight (UPLC-Q TOF) mass spectrometry. Chemometric tools were used to discover protein biomarkers for discrimination of wild and farmed Fenneropenaeus chinensis. The result showed that wild and farmed F. chinensis could be well discriminated through the protein biomarkers. This study may provide technical support for discrimination of wild and farmed F. chinensis.

Key words: Fenneropenaeus chinensis, wild, farmed, proteomics, chemometrics

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