食品科学 ›› 2019, Vol. 40 ›› Issue (9): 195-200.doi: 10.7506/spkx1002-6630-20180805-030

• 营养卫生 • 上一篇    下一篇

牦牛酥油支链脂肪酸对人乳腺癌细胞抑制的转录组学分析

袁锦莹1,孙万成1,罗毅皓1,*,丁建霞2   

  1. 1.青海大学农牧学院,青海 西宁 810016;2.青海省刚察县哈尔盖畜牧兽医站,青海 刚察 812300
  • 出版日期:2019-05-15 发布日期:2019-05-28
  • 基金资助:
    青海省科技厅基础研究项目(2018-ZJ-728;2017-ZJ-711)

Transcriptomic Analysis of Branched-Chain Fatty Acids from Yak Butter Inhibiting Proliferation of Human Breast Cancer Cells

YUAN Jinying1, SUN Wancheng1, LUO Yihao1,*, DING Jianxia2   

  1. 1. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China; 2. Station of Husbandry and Veterinary of Haergai, Gangcha County, Gangcha 812300, China
  • Online:2019-05-15 Published:2019-05-28

摘要: 为探讨从牦牛酥油中纯化的支链脂肪酸对人乳腺癌细胞株(SK-BR-3)生长抑制的影响,用牦牛酥油纯化的支链脂肪酸处理乳腺癌细胞24 h,进行转录组学分析;同时,采用流式细胞计数、噻唑蓝(methylthiazolyldiphenyl-tetrazolium bromide,MTT)、4’,6-二脒基-2-苯基吲哚(4’,6-diamidino-2-phenylindole,DAPI)染色实验进行验证。转录组学分析结果发现,与癌症、脂肪酸以及细胞凋亡相关的差异表达基因FOS、FADS2、TP53等被下调,揭示支链脂肪酸对人乳腺癌细胞生长有抑制作用。流式细胞计数、MTT、DAPI染色实验结果证明,牦牛酥油中的支链脂肪酸具有一定的细胞增殖抑制作用。因此,牦牛酥油中的支链脂肪酸对人乳腺癌细胞的生长有一定的抑制作用。

关键词: 牦牛酥油, 支链脂肪酸, 转录组学, 乳腺癌, 细胞凋亡

Abstract: The purpose was to investigate the effects of branched-chain fatty acids (BCFA) from yak butter on the proliferation of human breast cancer cell line (SK-BR-3). Free fatty acids (control) and BCFA from yak butter were applied to breast cancer cells for 24 h for transcriptomic analysis, and then the proliferation of cells was analyzed by flow cytometry, methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and 4’,6-diamidino-2-phenylindole (DAPI) staining. The transcriptome results revealed that the differentially expressed genes such as FOS, FADS2 and TP53 associated with cancer, fatty acids and apoptosis were down-regulated, implying the inhibitory effect of BCFA on the growth of human breast cancer cells, which was also confirmed by the results of flow cytometry, MTT analysis and DAPI staining.

Key words: yak butter, branched-chain fatty acids, transcriptomics, breast cancer, apoptosis

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