食品科学 ›› 2019, Vol. 40 ›› Issue (9): 207-212.doi: 10.7506/spkx1002-6630-20180504-041

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可溶性糖介导的荧光照射延缓鲜切西兰花衰老黄化

张翠翠,马亚丹,李林杰,庞凌云,詹丽娟*   

  1. 河南农业大学食品科学技术学院,河南 郑州 450002
  • 出版日期:2019-05-15 发布日期:2019-05-28
  • 基金资助:
    国家自然科学基金面上项目(31571903);河南省高校科技创新人才支持计划项目(14HASTIT025);河南省高等学校青年骨干教师培养计划项目(2017GGJS034)

Soluble Sugar-Mediated Yellowing Delay of Fresh-Cut Broccoli Florets (Brassica oleracea var. italica) Exposed to Fluorescence Irradiation

ZHANG Cuicui, MA Yadan, LI Linjie, PANG Lingyun, ZHAN Lijuan*   

  1. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China
  • Online:2019-05-15 Published:2019-05-28

摘要: 鲜切西兰花极易衰老黄化,严重影响其感官质量和商品价值。为减缓鲜切西兰花衰老黄化,延长贮藏期,研究荧光照射(光照)处理(24 μmol/(m2·s)、12 h/d)对鲜切西兰花贮藏期间(7 ℃、5 d)衰老黄化的影响。结果表明:和黑暗对照相比,光照处理能够显著维持鲜切西兰花叶绿素含量,降低黄化指数(P<0.05),同时诱导葡萄糖、蔗糖和果糖累积,与贮藏初期(第0天)相比,3 种糖在贮藏第5天分别增加了106%、73%和70%;进一步用0、40、80、120、160 mmol/L外源葡萄糖溶液对鲜切西兰花进行培养,结果发现在一定浓度范围(0~120 mmol/L)内,随着外源葡萄糖浓度增加,鲜切西兰花内源葡萄糖含量显著上升,黄化指数显著下降(P<0.05),而且黄化指数与外源葡萄糖浓度、内源葡萄糖含量呈极显著负相关(P<0.01),相关系数分别为-0.958和-0.878。因此,光照处理延缓鲜切西兰花黄化可能与其诱导的可溶性糖尤其是葡萄糖累积有关。

关键词: 衰老, 荧光, 保鲜, 叶绿素, 可溶性糖

Abstract: Fresh-cut broccoli florets are very susceptible to yellowing, which seriously affects their sensory quality and commercial value. In order to slow down the yellowing of fresh-cut broccoli florets and prolong the storage period, the effect of fluorescence irradiation (24 μmol/(m2·s), 12 h/d) on the senescence and yellowing of fresh-cut broccoli were studied by ion chromatography analysis. The results showed that light treatment significantly maintained the chlorophyll content and reduced the yellowing index of fresh-cut broccoli in comparison to dark control (P < 0.05). Meanwhile, light irradiation induced the accumulation of glucose, sucrose, and fructose, increasing by 106%, 73%, and 70% from day 0 to 5, respectively. The content of endogenous glucose in fresh-cut broccoli pronouncedly increased with increasing concentration of exogenous glucose up to 120 mmol/L. In addition, yellowing index markedly declined (P < 0.05), and it was significantly negatively correlated with exogenous and endogenous glucose concentration with correlation coefficients of ?0.958 and ?0.878, respectively (P < 0.01). Therefore, fluorescence irradiation can retard the senescence and yellowing of fresh-cut broccoli florets presumably inducing the accumulation of soluble sugars, especially glucose.

Key words: senescence, fluorescence, preservation, chlorophyll, soluble sugars

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