食品科学 ›› 2019, Vol. 40 ›› Issue (11): 182-187.doi: 10.7506/spkx1002-6630-20180312-122

• 营养卫生 • 上一篇    下一篇

胰岛素样生长因子-Ⅰ对乙酰水杨酸诱导小肠上皮细胞损伤的修复作用

王玥鑫,张立钢,安晶晶,马 明,刘 宁*   

  1. 东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江 哈尔滨 150000
  • 出版日期:2019-06-15 发布日期:2019-06-28
  • 基金资助:
    东北农业大学科技人才启动基金项目(2014RFQXJ071)

Protective Effect of Insulin-Like Growth Factor-I on Acetylsalicylic Acid-Induced Damage of Intestinal Epithelial Cells

WANG Yuexin, ZHANG Ligang, AN Jingjing, MA Ming, LIU Ning*   

  1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150000, China
  • Online:2019-06-15 Published:2019-06-28

摘要: 本实验研究胰岛素样生长因子-I(insulin-like growth factors,IGF-I)对乙酰水杨酸(acetylsalicylic acid,ASA)所致的小肠上皮细胞(intestinal epithelial cells,IEC-6)损伤的影响。通过构建ASA诱导IEC-6损伤模型,采用CCK-8法检测IGF-I对IEC-6损伤的修复作用;同时测定细胞培养液中乳酸脱氢酶(lactate dehydrogenase,LDH)活力,以及细胞中丙二醛(malondialdelyde,MDA)浓度、碱性磷酸酶(alkaline phosphatase,ALP)活力、细胞总蛋白质量浓度和Na+,K+-ATP酶活力。结果表明:与对照组相比,不同质量浓度的IGF-I均可以显著促进细胞的增殖(P<0.05);与损伤模型组相比,IGF-I能提高细胞存活率,降低细胞培养液中LDH活力,降低细胞ALP活力和MDA浓度,提高细胞总蛋白质量浓度,提高Na+,K+-ATP酶活力。IGF-I对ASA所致损伤的IEC-6可以促进增殖,抑制脂膜氧化,维持细胞形态的完整性,具有损伤修复作用。

关键词: 胰岛素样生长因子-I, 乙酰水杨酸, 小肠上皮细胞, 损伤, 增殖

Abstract: The present study was designed to investigate the repairing effect of insulin-like growth factors-I (IGF-I) on acetylsalicylic acid (ASA)-induced damage of intestinal epithelial cells (IEC-6). The cell damage model was induced by ASA. The CCK-8 assay was used to evaluate the proliferation of IEC-6. After being treated with ASA and IGF-I, the cells were collected for determining the activities of lactate dehydrogenase (LDH) and alkaline phosphatase (ALP), and the culture supernatant for analysis of Na+,K+-ATPase and alkaline phosphatase (ALP) and the contents of total protein and malondialdelyde (MDA). We found that compared with the control group, IGF-I at all concentrations tested promoted cell proliferation (P < 0.05). IGF-I increased the cell survival rate, decreased the LDH activity in the culture supernatant and reduced the ALP activity and MDA content in the cells, as well as elevated the total protein content and Na+,K+-ATPase activity. According to our results, IGF-I can promote the proliferation of IEC-6 damaged by ASA, inhibit membrane lipid peroxidation, maintain the morphological integrity and consequently repair cell damage.

Key words: insulin-like growth factors-I, acetylsalicylic acid, intestinal epithelial cells, injury, proliferation

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