食品科学 ›› 2019, Vol. 40 ›› Issue (12): 340-344.doi: 10.7506/spkx1002-6630-20180522-315

• 安全检测 • 上一篇    下一篇

电流-时间曲线法测定鱼露中的苯乙胺

赵晓娟1,2,周婵媛1,张 敏1,陈海光1,2,白卫东1,2   

  1. 1.仲恺农业工程学院轻工食品学院,广东 广州 510225;2.广州市广式传统食品加工与安全控制重点实验室,广东 广州 510225
  • 出版日期:2019-06-25 发布日期:2019-06-28
  • 基金资助:
    广东省科技计划项目(2016A040403116);广州市科技计划项目(201604020021;201604020050)

Determination of Phenethylamine in Fish Sauce by Amperometric Current-Time Curve Method

ZHAO Xiaojuan1,2, ZHOU Chanyuan1, ZHANG Min1, CHEN Haiguang1,2, BAI Weidong1,2   

  1. 1. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; 2. Guangzhou Key Laboratory of Traditional Cantonese Food Processing and Safety Control, Guangzhou 510225, China
  • Online:2019-06-25 Published:2019-06-28

摘要: 利用积分脉冲安培法和电流-时间(I-t)曲线法测定苯乙胺,对两种方法的测定条件分别进行优化,并比较两种方法的测定效果。结果表明:采用纳米金修饰金电极(AuNPs/AuE),在初始电位为0.1 V时,运用I-t曲线法测定苯乙胺具有良好的选择性和较高的灵敏度。苯乙胺的响应电流差值与苯乙胺浓度在4.13×10-8~4.72×10-6 mol/L范围内呈良好的线性关系,检出限为1.4×10-8 mol/L。用该法测定鱼露样品中苯乙胺的加标回收率在90.0%~110.8%之间。该法检测速度快、成本低、选择性好,适用于水产品及其制品中苯乙胺的测定。

关键词: 电流-时间曲线法, 积分脉冲安培法, 苯乙胺, 纳米金, 鱼露

Abstract: Phenethylamine was determined by the integrated pulsed amperometric (IPA) method and the amperometric current-time curve method. The detection conditions of the two methods were optimized, and the obtained results were compared with each other. The results showed that the amperometric I-t curve method had better selectivity and higher sensitivity to phenethylamine based on gold nanoparticles (AuNPs) modified gold electrode (AuE) at an initial potential of 0.1 V. The difference of response current had a good linear relationship with the concentration of phenethylamine in the range of 4.13 × 10-8–4.72 × 10-6 mol/L. The limit of detection (LOD) was 1.4 × 10-8 mol/L. The recovery of phenethylamine from spiked fish sauce was in the range of 90.0%–110.8%. This method was rapid, inexpensive, highly selective, and suitable for determination of phenethylamine in aquatic products.

Key words: amperometric I-t curve, integrated pulsed amperometric detection, phenethylamine, gold nanoparticles, fish sauce

中图分类号: