食品科学 ›› 2019, Vol. 40 ›› Issue (14): 130-137.doi: 10.7506/spkx1002-6630-20190123-292

• 生物工程 • 上一篇    下一篇

富硒短小芽孢杆菌D1-019的筛选与鉴定

孙 毓,肖益新,张 冉,王成华,刘小玲,赵谋明   

  1. 广西大学轻工与食品工程学院,广西 南宁 530004
  • 出版日期:2019-07-25 发布日期:2019-07-23
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0400105);广西创新驱动发展专项(AA17204075;桂科AA17202010-3)

Screening, Identification and Characterization of Selenium-Enriched Bacillus pumilus D1-019

SUN Yu, XIAO Yixin, ZHANG Ran, WANG Chenghua, LIU Xiaoling, ZHAO Mouming   

  1. College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China
  • Online:2019-07-25 Published:2019-07-23

摘要: 将无机硒耐受性筛选和“红硒法”筛选相结合,经过4 轮筛选,从前期分离的119 株来源于鸭食的菌株中,筛选到1 株富硒菌株D1-019。基于菌落形态观察、革兰氏染色、16S rDNA序列分析和系统发育树分析,D1-019菌株被鉴定为益生菌短小芽孢杆菌。采用正交试验设计方法,获得的最佳富硒条件为培养基初始pH 5,30 ℃培养48 h,培养6 h时加入质量浓度为20 μg/mL的亚硒酸钠。在该富硒条件下,短小芽孢杆菌D1-019对培养基中亚硒酸钠的转化率为100%,菌体有机硒含量为(1 327±113)mg/kg,优于已报道富硒微生物。结果表明,短小芽孢杆菌D1-019有潜力开发成为一个可应用于食品领域的新富硒益生菌源。

关键词: 短小芽孢杆菌, 有机硒, 富硒, 益生菌, 亚硒酸钠, 16S rDNA

Abstract: In this study, out of 119 bacterial isolates from duck foods, a novel selenium-enriched probiotic strain, designated as D1-019, was selected for its tolerance to selenite. This strain was obtained after four rounds of screening, which did not cause a red selenium phenomenon. Strain D1-019 was identified as Bacillus pumilus based on its colonial morphology, Gram staining, 16S rDNA analysis, and phylogenic tree analysis. Using an orthogonal experiment design, we obtained the optimum culture conditions for selenium enrichment as follows: pH 5, 30 ℃ for 48 h, and addition of 20 μg/mL selenite at 6 h of cultivation. Under these conditions, B. pumilus D1-019 eliminated selenite in the medium with a conversion rate of 100% and produced (1 327 ± 113) mg/kg of organic selenium, which was superior to the currently available seleniumenriched microbes. Our results indicated that B. pumilus D1-019 can be a promising candidate for selenium-enriched probiotics in the food industry.

Key words: Bacillus pumilus, organic selenium, selenium-enriched, probiotics, selenite, 16S rDNA

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