食品科学 ›› 2019, Vol. 40 ›› Issue (14): 144-152.doi: 10.7506/spkx1002-6630-20180725-315

• 生物工程 • 上一篇    下一篇

四川不同地区浓香型大曲微生物群落结构比较

吴树坤,谢 军,卫春会,刘燕梅,黄治国,万世旅,邓 杰   

  1. 1.四川理工学院 酿酒生物技术及应用四川省重点实验室,四川 自贡 643000;2.宜宾市产品质量监督检验所,四川 宜宾 644000
  • 出版日期:2019-07-25 发布日期:2019-07-23
  • 基金资助:
    四川省科技厅重点研发项目(2016SZ0074);四川省科技成果转化项目(2016CC0032);四川理工学院研究生创新基金资助项目(y2017049)

Comparison of Microbial Community Structure of Starter Cultures (Daqu) for Luzhou-Flavor Liquor in Different Regions of Sichuan

WU Shukun, XIE Jun, WEI Chunhui, LIU Yanmei, HUANG Zhiguo, WAN Shilü, DENG Jie   

  1. 1. Liquor-Making Biotechnology and Application Key Laboratory of Sichuan Province, Sichuan University of Science & Engineering, Zigong 643000, China; 2. Yibin Product Quality Supervision and Inspection Institute, Yibin 644000, China
  • Online:2019-07-25 Published:2019-07-23

摘要: 利用高通量测序技术分析四川不同地区浓香型大曲微生物群落结构差异。结果表明:大曲中优势细菌菌群为葡萄球菌属(Staphylococcus)、魏斯氏菌属(Weissella)、芽孢杆菌属(Bacillus)、乳杆菌属(Lactobacillus)、Kroppenstedtia、高温放线菌属(Thermoactinomyces)、糖多孢菌属(Saccharopolyspora)、短杆菌属(Brevibacterium),其中在品温较高的宜宾、泸州曲样中,魏斯氏菌属、高温放线菌属比例均高于遂宁曲样,而遂宁两个曲样的优势菌属均为葡萄球菌属和芽孢杆菌属,其中葡萄球菌属远高于宜宾和泸州曲样。另一方面,4 种曲样的真菌主要以嗜热真菌属(Thermomyces)、曲霉属(Aspergillus)、嗜热子囊菌属(Thermoascus)、丝衣霉属(Byssochlamys)、节担菌属(Wallemia)为主,在品温较高的宜宾、泸州曲样中,嗜热真菌属和嗜热子囊菌属为优势菌属,其比例远高于遂宁曲样,而遂宁两个曲样的优势菌属均为曲霉属,其比例远高于宜宾和泸州的曲样。遂宁1、遂宁2曲样的细菌和真菌多样性均高于宜宾和泸州曲样。通过主成分分析发现,遂宁1和遂宁2曲样微生物群落结构较相似,而宜宾和泸州曲样的微生物群落结构则更接近。

关键词: 大曲, 微生物群落, 高通量测序, 主成分分析

Abstract: In this study, we analyzed the microbial community structure of Luzhou-flavor liquor Daqu samples collected from different regions of Sichuan province by high throughput sequencing technology. The results showed that the dominant bacteria in the Daqu were Staphylococcus, Weissella, Bacillus, Lactobacillus, Kroppenstedtia, Thermoactinomyces, Saccharopolyspora, and Brevibacterium. The proportions of Thermoactinomyces and Weissella in Yibin Daqu and Luzhou Daqu, with higher temperature, were higher than those in two samples of Suining Daqu. The dominant strains in Suining Daqu were Staphylococcus and Bacillus, and Staphylococcus was much higher than in Yibin Daqu and Luzhou Daqu. On the other hand, the fungal communities in all four Daqu samples were predominated mainly by Thermomyces, Aspergillus, Thermoascus, Byssochlamys, and Wallemia. In Yibin and Luzhou samples, Thermomyces and Thermoascus were the dominant species, and their proportions were much higher than those in Suining Daqu. Aspergillus was the dominant species in Suining Daqu, and its proportion was much higher than those in Yibin Daqu and Luzhou Daqu. The diversities of bacteria and fungi in Suining Daqu were higher than in Yibin Daqu and Luzhou Daqu. Principal component analysis revealed that the microbial community structures of the Suining samples were more similar to each other while the microbial community structure of Yibin Daqu was more similar to Luzhou Daqu.

Key words: Daqu, microbial community, high-throughput sequencing, principal component analysis

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