食品科学 ›› 0, Vol. ›› Issue (): 0-0.

• 工艺技术 •    下一篇

采用超声-闪式联合法制备蓝莓花色苷提取物及其体内外抗肿瘤活性评价

薛宏坤,谭佳琪,刘成海,刘钗   

  1. 东北农业大学
  • 收稿日期:2019-04-12 修回日期:2020-01-06 出版日期:2020-03-25 发布日期:2020-03-23
  • 通讯作者: 刘钗 E-mail:liuchai@neau.edu.cn

Preparation of Blueberry Anthocyanin Extract by Ultrasound-Flash Method and Evaluation of Its Anti-Tumor Activity in Vivo and in Vitro

xuehongkun 2,   

  • Received:2019-04-12 Revised:2020-01-06 Online:2020-03-25 Published:2020-03-23

摘要: 以蓝莓为原料,采用超声-闪式联合法提取花色苷,探究超声功率、闪提转速、提取时间、料液比和乙醇体积分数对花色苷含量的影响;在单因素实验的基础上,利用遗传算法优化超声-闪式联合法提取蓝莓花色苷的工艺参数;体外实验采用MTT法和Annexin V-FITC/PI双标记染色法探究不同浓度花色苷提取物对S180细胞增殖和凋亡的影响;体内实验利用S180荷瘤小鼠模型和ELISA法探究花色苷提取物的抗肿瘤活性及其机制。结果表明:超声-闪式联合法提取蓝莓中花色苷的优化工艺参数为:提取时间10 min、超声强度450 W、闪提转速8400 r/min、料液比1:30 g/mL和乙醇体积分数68%,在此条件下花色苷含量为2.71±0.08 mg/g。体外实验发现花色苷提取物能有效抑制S180细胞增殖,诱导S180细胞凋亡,使细胞阻滞于S期。荷瘤小鼠体内抑制肿瘤实验表明:花色苷提取物低、中、高剂量组瘤体质量显著低于模型对照组(p<0.05),抑瘤率分别为26.36%、33.33%、46.51%;花色苷提取物低、中、高剂量组荷瘤小鼠血清中的IL-12和TNF-α含量显著高于模型对照组(p<0.05),而IL-10含量显著低于模型对照组(p<0.05)。综上超声-闪式联合提取法是一种高效的花色苷提取方式,所得的花色苷提取物可通过诱导细胞凋亡和增强机体免疫来抑制肿瘤生长。

关键词: 超声-闪式联合提取, 蓝莓, 花色苷, 工艺, 抗肿瘤活性

Abstract: Ultrasonic-flash extraction (UFE) was employed to extract the anthocyanins from blueberry. The effect of ultrasonic power, flash extraction speed, extraction time, solid-to-liquid ratio and ethanol concentration on the yield of anthocyanins were investigated. Genetic algorithm is used to optimize the process parameters of extraction considering single factor experiment. In vitro MTT and Annexin V-FITC/PI double label staining were employed to investigate the effects of anthocyanins extracts at different concentrations on proliferation and apoptosis of S180 cells. In vivo antitumor activity of anthocyanins extracts from was tested by mouse S180 tumor-bearing mice model and ELISA. The results showed that the optimum extracting parameters to achieve the highest the yield of anthocyanins of 2.71±0.08 mg/g from blueberry was obtained under an extraction time of 10 min, ultrasonic power of 450 W, flash extraction speed of 8400 r/min, solid-to-liquid ratio of 1:30 g/mL and ethanol concentration of 68%. In vitro experiments showed that anthocyanins extracts could remarkably inhibit the proliferation of S180 cells, induce the apoptosis of S180 cells, and block the cells in S phase. In vivo anti-tumor test in tumor-bearing mice showed that the mass of tumors in anthocyanins extracts low-dose, medium-dose and high- dose groups was significantly lower than that in model control group (p<0.05) , and the anti-tumor rates were 26.36%, 33.33% and 46.51%, respectively. The levels of IL-12 and TNF-α in serum of mice bearing anthocyanins extracts low-dose, medium-dose and high- dose groups were markedly higher than those of model control group (p<0.05), while the level of IL-10 were significantly lower than that of model control group (p<0.05) . In conclusion, the UFE is an efficient method for anthocyanins extraction, and the anthocyanins extracts can inhibit the growth of tumors by inducing apoptosis and enhancing immunity.

Key words: Ultrasonic-flash extraction, blueberry, anthocyanins, process, anti-tumor activity

中图分类号: