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冰温贮藏对杏果实冷害及活性氧代谢的影响

李亚玲1,崔宽波2,石玲1,祝兆帅2,李玲1,刘严1,朱璇3   

  1. 1. 新疆农业大学
    2. 新疆农业科学院农业机械化研究所
    3. 新疆农业大学食品科学与药学学院
  • 收稿日期:2019-04-26 修回日期:2020-02-17 出版日期:2020-04-15 发布日期:2020-04-20
  • 通讯作者: 朱璇 E-mail:zx9927@126.com
  • 基金资助:
    国家自然科学基金项目

Effect of Near Freezing Temperature storage on Chilling Injury and Active Oxygen Metabolism of Apricot Fruits

Ya-Ling LI 2, Zhaoshuai ZHU2,Ling LIYan LIu2,   

  • Received:2019-04-26 Revised:2020-02-17 Online:2020-04-15 Published:2020-04-20

摘要: 摘 要:以新疆库车小白杏作为试验材料,研究冰温贮藏对杏果实冷害及活性氧代谢的影响,分别贮藏于冰温(-1~-1.5 ℃)和冷藏(4~6 ℃、1~2 ℃)下49 d,每7 d取样,测定杏果实的冷害发病率、冷害指数和过氧化氢酶(catalase,CAT)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)活性、超氧阴离子自由基(superoxide anion,O2-?)产生速率;过氧化氢(hydrogen peroxide,H2O2)、丙二醛(malondialdehyde,MDA)含量及细胞膜透性。结果表明:与普通冷藏(4~6 ℃、1~2 ℃)相比,冰温贮藏可有效推迟冷害发病时间并降低冷害发病率,显著提高杏果实SOD的活性,延缓冷藏期间POD、 CAT活性的下降,抑制了H2O2含量和O2-?的产生速率,减少了丙二醛的积累和细胞膜透性的增加。表明冰温贮藏可提高杏果实的抗冷性和贮藏性。

关键词: 关键词:杏果实, 冰温贮藏, 冷害, 活性氧代谢

Abstract: Abstract: The purpose of this work was to investigate the effects of near freezing temperature storage on Chilling Injury and Active Oxygen Metabolism of Apricot Fruits.It were stored at Near Freezing Temperature (-1~-1.5 ℃) and Low Temperature (4~6 ℃、1~2 ℃) for 49 d respectively.The chilling injury incidence,chilling injury index,the activities of catalase (CAT),superoxide dismutase (SOD),peroxidase (POD),superoxide anion (O2-) production rate and the hydrogen peroxide (H2O2) ,malondialdehyde (MDA) content and membrane permeability of apricot fruits were measured at every seven days.The results showed that compared with the Low Temperature(4~6 ℃、1~2 ℃),near freezing temperature storage effectively delay the onset time of chilling injury and reduce the incidence of chilling injury,significantly increased the activities of SOD,and delay the decline of POD and CAT activity during cold storage.,inhibited the content of H2O2 and generation rate of O2-,retarded the increase of MDA and membrane permeability.Results indicated that near freezing temperature storage could improve the cold resistance and storage of apricot fruits .

Key words: Key words: apricot fruit, ice-temperature storage, chilling injury, active oxygen metabolism

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