大米球蛋白铜离子结合肽鉴定和螯合活性研究

食品科学 ›› 0, Vol. ›› Issue (): 0-0.

• 食品化学 • 下一篇

大米球蛋白铜离子结合肽鉴定和螯合活性研究

邢常瑞,初晨露,张晓云,汪金燕,鞠兴荣,袁建

南京财经大学

收稿日期:2019-05-21 修回日期:2019-10-08 出版日期:2019-12-25 发布日期:2019-12-24
通讯作者: 袁建 E-mail:13611513715@163.com
基金资助:
十三五国家重点研发计划项目

Identification of a copper-binding peptide from rice globulin hydrolysates and metal chelation activity evaluation

¹,Chen-Lu CHUChangrui Xing¹,Changrui Xing¹,

Received:2019-05-21 Revised:2019-10-08 Online:2019-12-25 Published:2019-12-24

摘要/Abstract

摘要： 为了分析大米球蛋白与金属元素的结合特性，通过分级提取方法得到了四种大米蛋白，并测定其金属元素含量。选取大米球蛋白经过胃蛋白酶酶解，酶解液通过超滤方式进行纯化，收集分子量小于3000的多肽溶液。将获得的水解液进行质谱检测，并通过软件分析得到23条多肽序列，分子量在725~1409之间。结合已报道的相关研究，分析得到多肽序列QGWSSSSE和YYGGEGSSSEQGY具有潜在金属螯合活性。通过人工合成这两条多肽序列，并选择EDTA、柠檬酸和金属硫蛋白作为参照物，比较了它们与Cu2+的螯合活性。结果发现YYGGEGSSSEQGY螯合Cu2+效果比QGWSSSSE好，与Cu2+螯合的IC20为5.40 mM，并且在低浓度范围与柠檬酸相当，比EDTA和金属硫蛋白差。进一步分析，这两条多肽与其他金属离子之间螯合活性发现，其与Fe2+结合更强。通过氨基酸分析，连续丝氨酸SS、SSS、和SSSS可能与铜的结合活性。

关键词: 大米球蛋白, 铜离子结合肽, 螯合活性

Abstract: In order to analyze the binding characteristics of rice globulin and metal elements, four types of rice proteins were obtained by sequential extraction method, and metal elements in them were determined. The rice globulin is selected to be hydrolyzed by pepsin, and the hydrolysates are purified by ultrafiltration. A mixed polypeptide solution with molecular weight less than 3000 was collected. The obtained hydrolysates was detected by mass spectrometry and 23 polypeptides were obtained based on software analysis, with molecular weight ranged from 725 to 1409. Based on reported literatures, polypeptides, QGWSSSSE and YYGGEGSSSEQGY, may have potential metal chelation activity. Their chelation activity with Cu2+ was compared using two synthetic polypeptides and compared with that of EDTA, citric acid and metallothionein. It was found that YYGGEGSSSEQGY has better Cu2+ chelation activity than QGWSSSSE. The IC20 of YYGGEGSSSEQGY was 5.40 mM, which was comparable to citric acid in low concentration range, and worse than EDTA and metallothionein. Further analysis, the chelation experiments between these two peptides and other metal ions showed that they could strongly bind to Fe2+. By amino acid analysis, continuous serine SS, SSS, and SSSS may be associated with copper binding activity.

Key words: rice globulin, copper-binding peptide, metal chelation activity

中图分类号:

TS211.4

邢常瑞初晨露张晓云汪金燕鞠兴荣袁建. 大米球蛋白铜离子结合肽鉴定和螯合活性研究[J]. 食品科学, 0, (): 0-0.

Chen-Lu CHU Changrui Xing Changrui Xing. Identification of a copper-binding peptide from rice globulin hydrolysates and metal chelation activity evaluation[J]. FOOD SCIENCE, 0, (): 0-0.

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大米球蛋白铜离子结合肽鉴定和螯合活性研究

Identification of a copper-binding peptide from rice globulin hydrolysates and metal chelation activity evaluation

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