关键词: 植物乳杆菌, 乙醇胁迫, 黏附, 膜脂肪酸

Abstract: Objective: To investigate the change in the adhesion capacity of Lactobacillus plantarum under ethanol stress and its physiological response against ethanol stress. Methods: L. plantarum was subjected to ethanol stress at different concentrations and its survival rate, cell membrane integrity and ultrastructural changes were determined. Human colon cancer HT-29 cells were used as an in vitro model to determine the adhesion capacity of this bacterium. The changes in cell membrane fatty acids were analyzed by gas chromatography-tandem mass spectrometry (GC-MS). Results: The survival rate of L. plantarum decreased gradually with the increase in ethanol concentration, and the cell membrane integrity and surface structure were damaged. In addition, the adhesion rate to HT-29 cells decreased, and the proportion of unsaturated fatty acids in the cell membrane increased. Conclusion: Ethanol stress can reduce the adhesion of L. plantarum to HT-29 cells. Fatty acids in the cell membrane and its integrity play an important role in the ethanol stress response of L. plantarum.

Key words: Lactobacilus planturam, ethanol stress, adhesion, membrane fatty acids