食品科学 ›› 2019, Vol. 40 ›› Issue (18): 89-94.doi: 10.7506/spkx1002-6630-20180901-003

• 生物工程 • 上一篇    下一篇

新疆喀什地区维吾尔族群体不同哺乳期母乳中乳杆菌的种群多样性

李小娟,倪永清,张艳   

  1. (石河子大学食品学院,新疆?石河子 832000)
  • 出版日期:2019-09-25 发布日期:2019-09-23
  • 基金资助:
    国家自然科学基金地区科学基金项目(31760446);石河子大学高层次人才启动项目(CZX201432R)

Diversity of Lactobacillus Species in Breast Milk Samples from Uygur Mothers at Different Lactation Stages in Kashi, Xinjiang

LI Xiaojuan, NI Yongqing, ZHANG Yan   

  1. (Food College, Shihezi University, Shihezi 832000, China)
  • Online:2019-09-25 Published:2019-09-23

摘要: 采用改良的MRS培养基进行菌株分离,多重聚合酶链式反应指纹图谱去重与16S rRNA同源性分析鉴定相结合,并通过MEGA V5.0建树对新疆喀什地区维吾尔族群体不同哺乳期母乳中乳杆菌的组成及变化进行研究。从45?个母乳样品中共分离出229?株乳酸菌,分别隶属于乳酸杆菌属(Lactobacillus)、肠球菌属(Enterococcus)和链球菌属(Streptococcus),其中96.1%的菌株隶属Lactobacillus spp.,分别为短乳杆菌(L. brevis)75?株、发酵乳杆菌(L. fermentum)82 株、口乳杆菌(L. ori)37?株、阴道乳杆菌(L. vaginalis)4?株和加氏乳杆菌(L. gasseri)22?株。其中L. vaginalis是比较罕见的菌种。新疆喀什地区维吾尔族群体母乳中乳杆菌种群组成丰富,不同哺乳期母乳中乳杆菌变化趋势明显;成熟乳样品中分离的乳杆菌种群结构相对初乳和晚乳的要复杂,其中5?d~5?个月哺乳期的母乳中乳杆菌种群结构最为复杂,为母乳中益生菌资源的开发提供依据。

关键词: 乳杆菌, 母乳, 种群, 多样性, 指纹图谱

Abstract: The Lactobacillus composition of breast milk samples from Uygur mothers at different stages of lactation in Kashi, Xinjiang was comparatively investigated by strain isolation using modified MRS medium, repetitive polymerase chain reaction (rep-PCR) fingerprinting, homology analysis of the 16S rRNA sequence, and construction of a phylogenetic tree by means of MEGA V5.0. The results showed that a total of 229 Lactobacillus strains were isolated from 45 breast milk samples and they were identified as Lactobacillus, Enterococcus and Streptococcus, respectively. Of these isolates, 96.1% belonged to Lactobacillus spp., including L. brevis (75 strains), L. fermentum (82 strains), L. ori (37 strains), L. vaginalis (4 strains) and L. gasseri (22 strains). L. vaginalis is a rare bacterial species. The obtained data showed that the composition of Lactobacillus in the breast milk samples was diverse and varied among lactation stages. The population structure of Lactobacillus in mature milk samples was more complex than that of colostrum and late milk, and it was the most complex in breast milk samples between day 5 and month 5. This study provides the basis for the development of probiotics resources from breast milk.

Key words: Lactobacillus, breast milk, population, diversity, fingerprint

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