食品科学 ›› 2019, Vol. 40 ›› Issue (18): 223-228.doi: 10.7506/spkx1002-6630-20181022-230

• 成分分析 • 上一篇    下一篇

冠突散囊菌发酵对茶汤香气成分的影响

郑梦霞,李会娟,陈淑娜,赵真,刘馨秋,黎星辉,陈暄   

  1. (南京农业大学茶叶研究所,江苏?南京 210095)
  • 出版日期:2019-09-25 发布日期:2019-09-23
  • 基金资助:
    现代农业产业技术体系建设专项(CARS-23);江苏现代农业产业技术体系建设项目(JATS[2018]212); 江苏省大学生创业实践计划项目(201810307005P)

Effect of Fermentation with Eurotium cristatum on Aroma Components of Tea Infusions

ZHENG Mengxia, LI Huijuan, CHEN Shuna, ZHAO Zhen, LIU Xinqiu, LI Xinghui, CHEN Xuan   

  1. (Institute of Tea Science, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2019-09-25 Published:2019-09-23

摘要: 为探讨冠突散囊菌(Eurotium cristatum)发酵对茶叶提取液香气成分的影响,利用冠突散囊菌对中低档绿茶和红茶提取液进行发酵,采用顶空固相微萃取结合气相色谱-质谱法分别检测审评用样品以及提取、灭菌和发酵后样品的香气成分,分析香气成分的变化规律。结果表明,绿茶与红茶提取液经冠突散囊菌发酵新产生苯甲酸甲酯、异佛尔酮、对甲氧基苯乙烯、对乙基苯甲醛、香叶酸甲酯、3,4-二甲基苯甲酸甲酯6?种化合物。同时,绿茶茶汤经冠突散囊菌发酵后,芳樟醇、芳樟醇氧化物I、芳樟醇氧化物II、苯乙酸甲酯、α-松油醇、反-2-癸烯醛和α-紫罗酮分别增长了1.30、2.15、2.04、0.29、3.18、0.10?倍和2.08?倍,且香气种类与含量均上升。但红茶茶汤经冠突散囊菌发酵后大部分香气物质含量下降,其中苯乙醇、反-2-壬烯醛、2-苯基巴豆醛、顺柠檬醛、4-甲基-2-苯基-2-戊烯醛、月桂醛、肉豆蔻醛等未检出,表明冠突散囊菌发酵红茶会带来香气总量的损耗,与红茶相比,低档绿茶更适合使用冠突散囊菌开发发酵型饮料。

关键词: 冠突散囊菌, 低档茶, 液体发酵, 香气成分, 气相色谱-质谱法

Abstract: The aroma components of green tea and black tea infusions were quantified by headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS) before and after fermentation with Eurotium cristatum. It was found that benzoic acid methyl ester, isophorone, methoxy styrene, ethyl benzene, formaldehyde, sweet folic acid methyl ester, and 3,4-dimethyl methyl benzoic acid were detected in the fermented tea infusions, but not in the fresh ones. In the green tea infusion, the concentrations of linalool, linalool oxide I, linalool oxide II, methyl phenylacetate, α-terpineol, trans-2-decylolefine aldehyde, and α-ionone were elevated by 2.30, 3.15, 3.04, 1.29, 4.18, 1.10 and 3.08, respectively after the fermentation, and the number and amount of aroma components increased similarly. In contrast, for the black tea infusion, the majority of the aroma compounds decreased and phenethyl alcohol, trans-2-nonenal, 2-phenylcrotonaldehyde, cis-citral, 4-methyl-2-phenyl-2-pentenal, lauraldehyde and tetradecanal were undetectable after the fermentation, indicating a loss of the total amount of aroma components. Compared to black tea, low-end green tea was more suitable for the processing of fermented beverage with E. cristatum.

Key words: Eurotium cristatum, low-grade tea, liquid-state fermentation, aroma compounds, gas chromatography-mass spectrometry

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