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• 营养卫生 •    下一篇

乳铁蛋白对三唑酮诱导骨髓间充质干细胞损伤的修复作用

谢银丹,赵添玉,许岩,任皓威,安晶晶,刘宁   

  1. 东北农业大学
  • 收稿日期:2019-07-19 修回日期:2020-05-27 出版日期:2020-07-15 发布日期:2020-07-18
  • 通讯作者: 刘宁 E-mail:ningliuneau@outlook.com

Repair Effect of Lactoferrin on Triadimefon Induced Damage of Bone Marrow Mesenchymal Stem Cells

1, 1,Yan XUHaowei REN1, Ning Liu   

  • Received:2019-07-19 Revised:2020-05-27 Online:2020-07-15 Published:2020-07-18
  • Contact: Ning Liu E-mail:ningliuneau@outlook.com

摘要: 本文研究乳铁蛋白(lactoferrin, Lf)对三唑酮(triadimefon, TDF)所致的骨髓间充质干细胞(bone marrow mesenchymal stem cells, BMSCs)损伤的影响。通过构建三唑酮诱导BMSCs损伤模型,采用CCK-8法检测Lf对BMSCs损伤的修复作用;酶联免疫法分析成骨过程中碱性磷酸酶(alkaline phosphatase, ALP)、骨钙素(osteocalcin, OCN)的表达;同时使用茜素红染色进行成骨分化能力检测,使用油红O染色进行成脂分化能力的检测;荧光定量PCR(RT-qPCR)分析成骨和成脂基因表达变化。结果表明,与空白对照组相比,不同质量浓度的Lf均可促进细胞增殖(P<0.05)。与损伤模型组相比,Lf能提高损伤BMSCs的细胞存活率,增加细胞ALP、OCN含量,增加细胞矿化结节的生成,减少脂滴的生成,增加成骨分化相关基因的相对表达,减少成脂分化相关基因的相对表达。Lf对三唑酮所致损伤的BMSCs能够促进增殖,使BMSCs向成骨方向进行分化,更好的促进新骨形成,具有损伤修复作用。

关键词: 三唑酮, 骨髓间充质干细胞(BMSCs), 乳铁蛋白, 分化, 修复

Abstract: The present study was designed to investigate the repairing effect of Lactoferrin (Lf) on triadimefon (TDF) induced damage of bone marrow mesenchymal stem cells (BMSCs). The cell damage model was induced by TDF. The CCK-8 assay was used to evaluate the proliferation of BMSCs. After being treated with TDF and Lf, the cells were collected for determining the activities of alkaline phosphatase (ALP) and osteocalcin (OCN), and the changes in osteogenesis and adipogenic gene expression. Detection of osteogenic differentiation ability using alizarin red staining and detection of adipogenic differentiation ability using oil red O staining. We found that compared with the blank control group, Lf at all concentrations tested promoted cell proliferation (P<0.05). Lf increased the cell survival rate, elevated the content of ALP and OCN in the cells, increased the formation of mineralized nodules, reduced the formation of lipid drople, as well as increased the relative expression of osteogenic differentiation-related genes, and decreased the relative expression of genes involved in adipogenic differentiation. According to our results, Lf can promote the proliferation of BMSCs damaged by TDF, differentiated the bone marrow mesenchymal stem cells into osteogenic direction, which can promote the formation of new bone better and consequently repair cell damage.

Key words: triadimefon, bone marrow mesenchymal stem cells (BMSCs), lactoferrin, differentiation, repair

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